Utilization of Fourier Transform Infrared Spectroscopy for Measurement of Organic Phosphorus and Bound Calcium in Cheddar Cheese

Typical buffering curve of a 1:39 (wt/wt) cheese:water dispersion (shown is the HLH treatment=high Ca and P, low lactose, and high S/M). The shaded area was used as the measure of buffering capacity of cheese.

Correlation between bound Ca and buffering peak area (n=167). HHH (●)=high Ca and P, high lactose, and high S/M; HHL (○)=high Ca and P, high lactose, and low S/M; HLH (▴)=high Ca and P, low lactose, and high S/M; HLL (▵)=high Ca and P, low lactose, and low S/M; LHH (■)=low Ca and P, high lactose, and high S/M; LHL (□)=low Ca and P, high lactose, and low S/M; LLH (♦)=low Ca and P, low lactose, and high S/M; LLL (♢)=low Ca and P, low lactose, and low S/M.

Unit area normalized infrared spectra used for characterizing the shifts. HHH=high Ca and P, high lactose, and high S/M; LLL=low Ca and P, low lactose, and low S/M.

A) Correlation coefficient (R) of different wavenumbers in the IR spectra with organic P values; B) corresponding partial least squares prediction model (n=216). HHH (●)=high Ca and P, high lactose, and high S/M; HHL (○)=high Ca and P, high lactose, and low S/M; HLH (▴)=high Ca and P, low lactose, and high S/M; HLL (▵)=high Ca and P, low lactose, and low S/M; LHH (■)=low Ca and P, high lactose, and high S/M; LHL (□)=low Ca and P, high lactose, and low S/M; LLH (♦)=low Ca and P, low lactose, and high S/M; LLL (♢)=low Ca and P, low lactose, and low S/M.

A) Correlation coefficient (R) of different wavenumbers in the IR spectra with bound Ca values; B) corresponding partial least squares prediction model (n=215). HHH (●)=high Ca and P, high lactose, and high S/M; HHL (○)=high Ca and P, high lactose, and low S/M; HLH (▴)=high Ca and P, low lactose, and high S/M; HLL (▵)=high Ca and P, low lactose, and low S/M; LHH (■)=low Ca and P, high lactose, and high S/M; LHL (□)=low Ca and P, high lactose, and low S/M; LLH (♦)=low Ca and P, low lactose, and high S/M; LLL (♢)=low Ca and P, low lactose, and low S/M.

A) Correlation coefficient (R) of different wavenumbers in the IR spectra with buffering peak areas; B) corresponding partial least squares prediction model (n=167). HHH (●)=high Ca and P, high lactose, and high S/M; HHL (○)=high Ca and P, high lactose, and low S/M; HLH (▴)=high Ca and P, low lactose, and high S/M; HLL (▵)=high Ca and P, low lactose, and low S/M; LHH (■)=low Ca and P, high lactose, and high S/M; LHL (□)=low Ca and P, high lactose, and low S/M; LLH (♦)=low Ca and P, low lactose, and high S/M; LLL (♢)=low Ca and P, low lactose, and low S/M.