Technical Note: A Rapid Pulsed-Field Gel Electrophoresis Method for Analysis of Bifidobacteria

Briczinski, E.P.; Roberts, R.F.

doi:10.3168/jds.S0022-0302(06)72315-3

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Volume 89, Issue 7 , Pages 2424-2427, July 2006

Technical Note: A Rapid Pulsed-Field Gel Electrophoresis Method for Analysis of Bifidobacteria

Department of Food Science, The Pennsylvania State University, University Park 16802

Received 4 October 2005; accepted 23 February 2006.

Abstract

Pulsed-field gel electrophoresis (PFGE) is a widely used and highly discriminatory molecular typing method that has been applied to bifidobacteria. However, published PFGE protocols used with bifidobacteria require between 5 and 7 d to complete. A rapid PFGE method was developed that can be completed within 24h.