Journal of Dairy Science
Volume 89, Issue 11 , Pages 4156-4162 , November 2006

Detection and Quantification of Capsular Exopolysaccharides from Streptococcus thermophilus Using Lectin Probes

  • G. Robitaille

      Affiliations

    • Food Research and Development Centre (FRDC), Agriculture and Agri-Food Canada, Saint-Hyacinthe, Quebec, Canada, J2S 8E3
    • Corresponding Author InformationCorresponding author.
    • ,
  • S. Moineau

      Affiliations

    • Département de biochimie et de microbiologie, Faculté des sciences et de génie, Université Laval, Québec City, Québec, Canada, G1K 7P4
    • Groupe de recherche en écologie buccale (GREB), Faculté de médecine dentaire, Université Laval, Québec City, Québec, Canada, G1K 7P4
    • Félix d’Hérelle Reference Centre for Bacterial Viruses, Université Laval, Québec City, Québec, Canada, G1K 7P4
    • ,
  • D. St-Gelais

      Affiliations

    • Food Research and Development Centre (FRDC), Agriculture and Agri-Food Canada, Saint-Hyacinthe, Quebec, Canada, J2S 8E3
    • ,
  • C. Vadeboncoeur

      Affiliations

    • Département de biochimie et de microbiologie, Faculté des sciences et de génie, Université Laval, Québec City, Québec, Canada, G1K 7P4
    • Groupe de recherche en écologie buccale (GREB), Faculté de médecine dentaire, Université Laval, Québec City, Québec, Canada, G1K 7P4
    • ,
  • M. Britten

      Affiliations

    • Food Research and Development Centre (FRDC), Agriculture and Agri-Food Canada, Saint-Hyacinthe, Quebec, Canada, J2S 8E3

Received 23 December 2005 ,Accepted 28 May 2006.

References 

  1. Ariga H, Urashima T, Michihata E, Ito M, Morizono N, Kimura T, et al. Extracellular polysaccharide from encapsulated Streptococcus salivarius subsp. thermophilus OR 901 isolated from commercial yogurt. J. Food Sci. 1992;57:625–628
  2. Broadbent JR, McMahon DJ, Oberg CJ, Welker DL. Use of exopolysaccharide-producing cultures to improve the functionality of low fat cheese. Int. Dairy J. 2001;11:433–439
  3. Broadbent JR, McMahon DJ, Welker DL, Oberg CJ, Moineau S. Biochemistry, genetics and applications of exopoly-saccharide production in Streptococcus thermophilus: A review. J. Dairy Sci. 2003;86:407–423
  4. Dabour N, LaPointe G, Benhamou N, Fliss I, Kheadr EE. Application of ruthenium red and colloidal gold-labeled lectin for the visualization of bacterial exopolysaccharides in Cheddar cheese matrix using transmission electron microscopy. Int. Dairy J. 2005;15:1044–1055
  5. Dabour N, Kheadr E, Benhamou N, Fliss I, LaPointe G. Improvement of texture and structure of reduced-fat cheddar cheese by exopolysaccharide-producing Lactococci. J. Dairy Sci. 2006;89:95–110
  6. Degeest B, Vaningelgem F, de Vuyst L. Microbial physiology, fermentation kinetics, and process engineering of heteropoly-saccharide production by lactic acid bacteria. Int. Dairy J. 2001;11:747–757
  7. Deveau H, Moineau S. Use of RFLP to characterize Lacto-coccus lactis strains producing exopolysaccharide. J. Dairy Sci. 2003;86:1472–1475
  8. de Vuyst L, de Vin F, Vaningelgem F, Degeest B. Recent developments in the biosynthesis and applications of heteropoly-saccharides from lactic acid bacteria. Int. Dairy J. 2001;11:687–707
  9. Folkenberg DM, Dejmek P, Skriver A, Ipsen R. Relation between sensory texture properties and exopolysaccharide distribution in set and in stirred yoghurts produced with different starter cultures. J. Texture Stud. 2005;36:174–189
  10. Gancel F, Novel G. Exopolysaccharide production by Streptococcus salivarius ssp. thermophilus cultures. 1. Conditions of production. J. Dairy Sci. 1994;77:685–688
  11. Goh KKT, Haisman DR, Singh H. Development of an improved procedure for isolation and purification of exopolysaccharides produced by Lactobacillus delbrueckii subsp. bulgaricus. Appl. Microbiol. Biotechnol. 2005;67:202–208
  12. Hassan AN, Frank JF, Farmer MA, Schmidt KA, Shalabi SI. Observation of encapsulated lactic acid bacteria using confocal scanning laser microscopy. J. Dairy Sci. 1995;78:2624–2628
  13. Hassan AN, Frank JF, Shalabi SI. Factors affecting capsule size and production by lactic acid bacteria used as dairy starter cultures. Int. J. Food Microbiol. 2001;64:199–203
  14. Hassan AN, Frank JF, Qvist KB. Direct observation of bacterial exopolysaccharides in dairy products using confocal scanning laser microscopy. J. Dairy Sci. 2000;85:1705–1708
  15. Holm C, Jespersen LA. Flow-cytometric gram-staining technique for milk-associated bacteria. Appl. Environ. Microbiol. 2003;69:2857–2863
  16. Kellens JTC, Jacobs JA, Peumans WJ, Stobberingh EE. The agglutination of β-haemolytic streptococci by lectins. J. Med. Microbiol. 1993;39:440–445
  17. Lévesque C, Duplessis M, Labonté J, Labrie S, Fremaux C, Tremblay D, et al. Genomic organization and molecular analysis of the virulent bacteriophage 2972 infecting an exopolysaccharide-producing Streptococcus thermophilus strain. Appl. Environ. Microbiol. 2005;71:4057–4068
  18. Low D, Ahlgren JA, Horne D, McMahon DJ, Oberg CJ, Broadbent JR. Role of Streptococcus thermophilus MR-1C capsular exopolysaccharide in cheese moisture retention. Appl. Environ. Microbiol. 1998;64:2147–2151
  19. Neu TR, Swerhone GDW, Lawrence JR. Assessment of lectin-binding analysis for in situ detection of glycoconjugates in biofilm systems. Microbiology. 2001;147:299–313
  20. Petersen BL, Dave RI, McMahon DJ, Oberg CJ, Broadbent JR. Influence of capsular and ropy exopolysaccharide-producing Streptococcus thermophilus on Mozzarella cheese and cheese whey. J. Dairy Sci. 2000;83:1952–1956
  21. Rimada PS, Abraham AG. Comparative study of different methodologies to determine the exopolysaccharide produced by kefir grains in milk and whey. Lait. 2003;83:79–87
  22. Ruas-Madiedo P, de los Reyes-Gavilan CG. Methods for the screening, isolation, and characterization of exopolysaccharides produced by lactic acid bacteria. J. Dairy Sci. 2005;88:843–856
  23. Sizemore RK, Cadwell JJ, Kendrick AS. Alternate gram staining technique using fluorescent lectin. Appl. Environ. Microbiol. 1990;56:2245–2247
  24. Terzaghi BE, Sandine WE. Improved medium for lactic streptococci and their bacteriophages. Appl. Microbiol. 1975;20:807–813
  25. Tremblay DM, Moineau S. Complete genomic sequence of the lytic bacteriophage DT1 of Streptococcus thermophilus. Virology. 1999;255:63–76
  26. Vaningelgem F, Zamfir M, Mozzi F, Adriany T, Vancanneyt M, Swings J, et al. Biodiversity of exopolysaccharides produced by Streptococcus thermophilus strains is reflected in their production and their molecular and functional characteristics. Appl. Environ. Microbiol. 2004;70:900–912
  27. Whitfield C, Paiment A. Biosynthesis and assembly of Group 1 capsular polysaccharides in Escherichia coli and related extracellular polysaccharides in other bacteria. Carbohydrate Res. 2003;338:2491–2502

PII: S0022-0302(06)72460-2

doi: 10.3168/jds.S0022-0302(06)72460-2

Journal of Dairy Science
Volume 89, Issue 11 , Pages 4156-4162 , November 2006

Article Tools

* Image Usage & Resolution