Immunomodulatory Effects of Polysaccharides Produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1

Fractionation of extracellular polysaccharides (EPS) produced by Lactobacillus bulgaricus OLL1073R-1 by anion-exchange chromatography. The eluate (approximately 7.8 mL/fraction) was monitored for proteins at 280nm (●) and for neutral sugars at 490nm (○) by the phenol-H₂SO₄ method. The dashed line indicates the concentration of NaCl; APS = acidic EPS; NPS = neutral EPS.

Elution profile of acidic extracellular polysaccharides (APS) by gel filtration chromatography. The eluate (approximately 7.8 mL/fraction) was monitored for proteins at 280nm (●) and for sugar at 490nm (○) by the phenol-H₂SO₄ method. L-APS and H-APS indicated low and high molecular weight APS, respectively.

Effect of extracellular polysaccharides (EPS) produced by Lactobacillus bulgaricus OLL1073R-1 on IFN-γ production from mouse splenocytes. C3H/HeJ mouse splenocytes were incubated with high molecular weight acidic EPS (H-APS,●) or neutral EPS (NPS, ▴) for 72h. Each bar represents the mean value and SD of triplicate samples. An asterisk (*) indicates P<0.05 compared with the control using the Dunnett's test.

Effect of orally administered high molecular weight acidic extracellular polysaccharides (H-APS) produced by Lactobacillus bulgaricus OLL1073R-1 on natural killer cell activity in mouse splenocytes. Splenocytes from BALB/c mice administered H-APS for 3 wk were examined. Values are means of 10 mice per group. An asterisk (*) indicates P<0.05 compared with the control using the Dunnett's test.

Effect of orally administered yogurt fermented with Lactobacillus bulgaricus OLL1073R-1 on natural killer cell activity in splenocytes of BALB/c mice. Splenocytes from mice administered yogurt fermented with L. bulgaricus OLL1073R-1 and Streptococcus thermophilus OLS3059 (1073R-1 yogurt) for 4 wk were examined. Control yogurt was fermented with L. bulgaricus OLL1256 and S. thermophilus OLS3295. Values are means of 8 mice per group. An asterisk (*) indicates P<0.05 compared with the control using the Student's t-test.

Effect of orally administered yogurt fermented with Lactobacillus bulgaricus OLL1073R-1 on the production of IFN-γ and IL-4 by BALB/c mouse splenocytes. Splenocytes from mice administered yogurt fermented with L. bulgaricus OLL1073R-1 and Streptococcus thermophilus OLS3059 (1073R-1 yogurt) for 4 wk were examined. Control yogurt was fermented with L. bulgaricus OLL1256 and S. thermophilus OLS3295. Splenocytes were incubated with concanavalin A for 72h. Values are means of 8 mice per group.

Effect of extracellular polysaccharides (EPS) purified from yogurt fermented with Lactobacillus bulgaricus OLL1073R-1 on IFN-γ production from splenocytes of C3H/HeJ mice. Splenocytes were incubated with concanavalin A for 72h. Each bar represents the mean value and SD of 5 samples. An asterisk (*) indicates P<0.05 compared with the control using the Dunnett's test.