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Volume 90, Issue 3 , Pages 1176-1185 , March 2007

Phenotypic and Genetic Antibiogram of Methicillin-Resistant Staphylococci Isolated from Bovine Mastitis in Korea

J.-S. Moon
- Department of Bacteriology, National Veterinary Research and Quarantine Service, Anyang, Gyeonggi-do, Republic of Korea
A.-R. Lee
- Department of Biology, Sangmyung University, Seoul, Republic of Korea
H.-M. Kang
- Department of Bacteriology, National Veterinary Research and Quarantine Service, Anyang, Gyeonggi-do, Republic of Korea
E.-S. Lee
- Department of Bacteriology, National Veterinary Research and Quarantine Service, Anyang, Gyeonggi-do, Republic of Korea
M.-N. Kim
- Department of Biology, Sangmyung University, Seoul, Republic of Korea
Y.H. Paik
- KRF Zoonotic Disease Priority Research Institute, Department of Microbiology, College of Veterinary Medicine and the BK21 Program for Veterinary Science, Seoul National University, Seoul, Republic of Korea
Y.H. Park
- KRF Zoonotic Disease Priority Research Institute, Department of Microbiology, College of Veterinary Medicine and the BK21 Program for Veterinary Science, Seoul National University, Seoul, Republic of Korea
Y.-S. Joo
- Department of Bacteriology, National Veterinary Research and Quarantine Service, Anyang, Gyeonggi-do, Republic of Korea
H.C. Koo
- KRF Zoonotic Disease Priority Research Institute, Department of Microbiology, College of Veterinary Medicine and the BK21 Program for Veterinary Science, Seoul National University, Seoul, Republic of Korea
- Corresponding author.

Received 25 April 2006 ,Accepted 10 September 2006.

Detection of coagulase gene (coa) and mecA gene by multiplex PCR from methicillin-resistant Staphylococcus aureus (MRSA). Lane 1=100-bp molecular weight ladder; lanes 2 to 7=isolated MRSA; lane 8=Stap

Detection of coagulase gene (coa) and mecA gene by multiplex PCR from methicillin-resistant Staphylococcus aureus (MRSA). Lane 1=100-bp molecular weight ladder; lanes 2 to 7=isolated MRSA; lane 8=Staph. aureus ATCC 43300 (MRSA); lane 9=isolated methicillin-susceptible Staph. aureus (MSSA); lane 10=Staph. aureus ATCC 25923 (MSSA).
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The coagulase gene of Staphylococcus aureus was amplified and digested with AluI restriction enzyme. Lane 1=pGEM DNA marker (Promega Corp, Madison, WI); lanes 2 to 11=PCR-RFLP patterns A to J, respect

The coagulase gene of Staphylococcus aureus was amplified and digested with AluI restriction enzyme. Lane 1=pGEM DNA marker (Promega Corp, Madison, WI); lanes 2 to 11=PCR-RFLP patterns A to J, respectively.
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