« Previous Next » Journal of Dairy Science
Volume 90, Issue 4 , Pages 1653-1661 , April 2007

Biofilm Ecology of Wooden Shelves Used in Ripening the French Raw Milk Smear Cheese Reblochon de Savoie

C. Mariani
- Université Claude Bernard Lyon 1, Laboratoire de Recherche en Génie Industriel Alimentaire, EA 3733, IUT A Département Génie Biologique, Bourg en Bresse, F-01060 France
- Unité Mixte de Recherche 763-UBHM, Institut National de la Recherche Agronomique et Ecole Nationale Supérieure des Industries Agricoles et Alimentaires, Massy, F-91744 France
- Institut Technique Français des Fromages, La Roche sur Foron, F-74801 France
R. Briandet
- Unité Mixte de Recherche 763-UBHM, Institut National de la Recherche Agronomique et Ecole Nationale Supérieure des Industries Agricoles et Alimentaires, Massy, F-91744 France
J.-F. Chamba
- Institut Technique Français des Fromages, La Roche sur Foron, F-74801 France
E. Notz
- Institut Technique Français des Fromages, La Roche sur Foron, F-74801 France
A. Carnet-Pantiez
- Université Claude Bernard Lyon 1, Laboratoire de Recherche en Génie Industriel Alimentaire, EA 3733, IUT A Département Génie Biologique, Bourg en Bresse, F-01060 France
R.N. Eyoug
- Université Claude Bernard Lyon 1, Laboratoire de Recherche en Génie Industriel Alimentaire, EA 3733, IUT A Département Génie Biologique, Bourg en Bresse, F-01060 France
N. Oulahal
- Université Claude Bernard Lyon 1, Laboratoire de Recherche en Génie Industriel Alimentaire, EA 3733, IUT A Département Génie Biologique, Bourg en Bresse, F-01060 France
- Corresponding author.

Received 31 March 2006 ,Accepted 24 November 2006.

Effect of age of wooden shelf on the means of the counts of different groups of microorganisms on wooden shelves (log₁₀(cfu/cm²). Error bars indicate confidence interval (P<0.05). Young=less than 4

Effect of age of wooden shelf on the means of the counts of different groups of microorganisms on wooden shelves (log₁₀(cfu/cm²). Error bars indicate confidence interval (P<0.05). Young=less than 4 yr old; medium=from 5 to 8 yr old; old=more than 8 yr old. MC=micrococci-corynebacteria; FH=facultatively heterofermentative.
- Full-Size Image
- Download to PowerPoint
Effect of the cheese producing farmhouses (A to H) on the means of the total bacterial counts, the micrococci-corynebacteria, and the yeast-mould on wooden shelves (log10(cfu/cm2). Error bars indicate

Effect of the cheese producing farmhouses (A to H) on the means of the total bacterial counts, the micrococci-corynebacteria, and the yeast-mould on wooden shelves (log₁₀(cfu/cm²). Error bars indicate confidence interval (P<0.05). ^a–cLetters correspond to statistically different groups.
- Full-Size Image
- Download to PowerPoint
Effect of season on the means of the total bacterial counts and the 7 different groups of microorganisms on wooden shelves (log10(cfu/cm2). Error bars indicate confidence interval (P<0.05). Summer:

Effect of season on the means of the total bacterial counts and the 7 different groups of microorganisms on wooden shelves (log₁₀(cfu/cm²). Error bars indicate confidence interval (P<0.05). Summer: samples collected from June to July. Autumn: samples collected from October to November. MC=micrococci-corynebacteria; FH=facultatively heterofermentative. ***P<0.001; *P<0.05.
- Full-Size Image
- Download to PowerPoint
Scanning electron microscopy of the surface of a ripening shelf. A (×300), ripening biofilm does not cover all the surface of the shelf. B (×1,500), bacteria are located in the cracks. C (×4,000) and

Scanning electron microscopy of the surface of a ripening shelf. A (×300), ripening biofilm does not cover all the surface of the shelf. B (×1,500), bacteria are located in the cracks. C (×4,000) and D (×6,000), details of Geotrichum candidum (cylinders) and bacteria.
- Full-Size Image
- Download to PowerPoint