In Vitro Assessment of the Gastrointestinal Transit Tolerance of Taxonomic Reference Strains from Human Origin and Probiotic Product Isolates of Bifidobacterium

Survival behavior of probiotic product isolate Bifidobacterium breve LM 646 compared with B. breve reference strains (stationary phase cells) over a 180-min incubation period in simulated gastric juice. For each strain, trendlines were calculated based on the log of the mean ratio of green to red fluorescence emission (% ratio_G/R) values of 4 time points (0, 1, 90, and 180min), which are expressed as the mean±standard deviation (bars indicated when >0.1) of 3 measurements. Comparison and statistical analysis were based on the slopes of these trendlines (slope range for B. breve reference strains: −0.0028 to −0.0074 and B. breve LM 646: −0.0001).