Profile of Gelatinolytic Capacity of Raw Goat Milk and the Implications for Milk Quality

Semiquantitative zymography for human plasmin standard on fibrin SDS-PAGE (A) and human gelatinase standard on gelatin SDS-PAGE (B) loaded at the indicated nanogram levels. Panels C and D show a representative goat milk sample on regular SDS-PAGE (C) and on gelatin SDS-PAGE (D) loaded at the indicated microgram protein levels. LF = lactoferrin, IGh = immunoglobulin heavy chain.

Monthly profiles of SCC and proteases in milk from 2 individual goats: A) Microscopic SCC, B) fibrin SDS-PAGE, and C) gelatin SDS-PAGE were displayed as times relative to milk stasis.

Weekly profiles of somatic cell counts and proteases in the last-month milk of 4 individual goats. A) Microscopic SCC and B) gelatin SDS-PAGE were displayed as times relative to milk stasis.

Weekly profiles of SCC and proteases in udder secretions of two dried goats. A) Microscopic SCC, B) fibrin SDS-PAGE, and C) gelatin SDS-PAGE were displayed as times relative to milk stasis.

Distribution of gelatinolytic capacities of goat milk, as well as microscopic somatic cell counts (MSCC), for experimental goats according to the corresponding time relative to milk stasis.

Distribution of gelatinolytic capacities of goat milk for experimental goats according to the corresponding microscopic somatic cell counts (MSCC).

Aligned histograms of percentages of ultracentrifuge-precipitable casein of total milk protein (upper panel) and proteinase capacity (lower panel) of raw milk collected from 23 goats.

Distribution of casein ratio of total milk protein of experimental goats according to the respective capacity of proteinases of goat milk.