Characteristics of Reduced Fat Milks As Influenced By the Incorporation of Folic Acid

Standard curve of folic acid concentrations.

Standard curve of folate-binding protein (FBP) concentrations.

Mean (±SE) concentration (mg/L) of folic acid in reduced fat milks at 25, 50, 75, and 100% of recommended daily allowance added before or after pasteurization.

Peak area (HPLC) of reduced fat milks fortified with folic acid at 25% (panels A and B) and 100% (panels C and D) of recommended daily allowance (RDA) before (A and C) and after (B and D) pasteurization at d 1.

Mean (±SE) concentrations (nmol/L) of folate-binding protein (FBP) in reduced fat milks with folic acid at 25, 50, 75, and 100% of recommended daily allowance (RDA) added before or after pasteurization.

Polyacrylamide gel electrophoresis of reduced fat milks with folic acid added before and after pasteurization at 75 and 100% of recommended daily allowance (RDA) on d 21. Std = molecular weight standard; lane 1 = folate-binding protein standard; lane 2 = raw milk; lane 3 = control; lane 4 = folic acid added at 75% RDA before pasteurization; lane 5 = folic acid added at 75% RDA after pasteurization; lane 6 = folic acid added at 100% RDA before pasteurization; lane 7 = folic acid added at 100% RDA after pasteurization.

Mean SPC of reduced fat milks fortified with folic acid at 25, 50, 75, and 100% of recommended daily allowance (RDA) added before or after pasteurization.