Effect of Calcium on the Physical Properties of Stirred Probiotic Yogurt

Abstract 

The effect of calcium on the viscosity, firmness, and smoothness, as determined by extent of nodulation, of stirred probiotic yogurt produced by bacterial fermentation was investigated. Standardized milk for yogurt manufacture was prepared, and calcium was added or removed from the system. Calcium was added as Ca²⁺ in the form of CaCl₂ (up to 13.6mM) or nonionic calcium as Gadocal-K (calcium potassium citrate; up to 49.8mM). Calcium was removed by chelating with sodium citrate (up to 16mM) or by cation exchange with Amberlite IR-120 plus (sodium form) resin (up to 10g/L). Calcium chloride and sodium citrate were added either before or after heat treatment of milk, and nonionic calcium was added before heat treatment. Calcium removal by ion exchange was performed before heat treatment. Neither Ca²⁺ addition nor removal by chelation with citrate resulted in stirred yogurt with viscosity, firmness, and smoothness superior to those of the control yogurt, whereas addition of 49.8mM nonionic calcium and removal of calcium (5.6mM or ∼10% of total calcium) by cation exchange improved the firmness and viscosity without affecting yogurt smoothness. The study identified Gadocal-K as a possible source of calcium fortification of stirred yogurt without loss of texture.

Key words: calcium, stirred yogurt, nodulation, viscosity