Journal of Dairy Science
Volume 91, Issue 10 , Pages 3779-3786 , October 2008

Evaluation of Molecular Methods for the Detection of Brucella Species in Water Buffalo Milk

  • C. Marianelli

      Affiliations

    • Dipartimento di Sanità Pubblica Veterinaria e Sicurezza Alimentare, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
    • Corresponding Author InformationCorresponding author.
  • ,
  • A. Martucciello

      Affiliations

    • Istituto Zooprofilattico Sperimentale del Mezzogiorno, Sezione Diagnostica Provinciale di Salerno, Centro di Referenza Nazionale sull’igiene e le tecnologie dell’allevamento e delle produzioni bufaline, Via delle Calabrie 27, 84132 Fuorni-Salerno, Italy
  • ,
  • M. Tarantino

      Affiliations

    • Dipartimento di Sanità Pubblica Veterinaria e Sicurezza Alimentare, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
  • ,
  • R. Vecchio

      Affiliations

    • Istituto Zooprofilattico Sperimentale del Mezzogiorno, Sezione Diagnostica Provinciale di Salerno, Centro di Referenza Nazionale sull’igiene e le tecnologie dell’allevamento e delle produzioni bufaline, Via delle Calabrie 27, 84132 Fuorni-Salerno, Italy
  • ,
  • G. Iovane

      Affiliations

    • Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Della Salute 2, 80055 Portici-Napoli, Italy
  • ,
  • G. Galiero

      Affiliations

    • Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Della Salute 2, 80055 Portici-Napoli, Italy

Received 4 April 2008 ,Accepted 6 June 2008.

References 

  1. Alton GG, Jones LM, Angus RD, Verger JM. Techniques for the Brucellosis Laboratory. Paris, France: Institut National de la Recherche Agronomique Publications; 1988;
  2. Baily GG, Kraahn JB, Drasar BS, Stoker NG. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J. Trop. Med. Hyg. 1992;95:271–275
  3. Blasco JM, Marín C, Jiménez de Bagués M, Barberán M, Hernández A, Molina L, et al. Evaluation of allergic and serological tests for diagnosing Brucella melitensis infection in sheep. J. Clin. Microbiol. 1994;32:1835–1840
  4. Borriello G, Capparelli R, Bianco M, Fenizia D, Alfano F, Capuano F, et al. Genetic resistance to Brucella abortus in the water buffalo (Bubalus bubalis). Infect. Immun. 2006;74:2115–2120
  5. Corbel MJ, MacMillan AP. In:  Collier L,  Balows A,  Sussman M editor. Brucellosis, in Topley & Wilson's Microbiology and Microbial Infections. London, UK: Georgina Bentliff; 1998;p. 819–847
  6. Cremonesi P, Castiglioni B, Malferrari G, Biunno I, Vimercati C, Moroni P, et al. Technical note: Improved method for rapid DNA extraction of mastitis pathogens directly from milk. J. Dairy Sci. 2006;89:163–169
  7. Diaz-Aparicio E, Marin C, Alonso-Urmeneta B, Aragon V, Perez-Ortiz S, Pardo M, et al. Evaluation of serological tests for diagnosis of Brucella melitensis infection of goats. J. Clin. Microbiol. 1994;32:1159–1165
  8. Dobson A, Meagher M. The population dynamics of brucellosis in the Yellowstone National Park. Ecology. 1996;77:1026–1036
  9. Enright FM. The pathogenesis and pathobiology of Brucella infection in domestic animals. In:  Nielsen K,  Duncan JR editor. Chapter 12 in Animal Brucellosis. Boca Raton, FL: CRS Press; 1990;
  10. Fekete A, Bantle JA, Halling SM, Sanborn MR. Preliminary development of a diagnostic test for Brucella using polymerase chain reaction. J. Appl. Bacteriol. 1990;69:216–227
  11. Godfroid J, Saegerman C, Wellemans V, Walravens K, Letesson JJ, Tibor A, et al. How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Vet. Microbiol. 2002;90:461–477
  12. Guarino A, Serpe L, Fusco G, Scaramuzzo A, Gallo P. Detection of Brucella species in buffalo whole blood by gene-specific PCR. Vet. Rec. 2000;147:634–636
  13. Hamdy MER, Amin AS. Detection of Brucella species in the milk of infected cattle, sheep, goats and camels by PCR. Vet. J. 2002;163:299–305
  14. Herman L, De Ridder H. Identification of Brucella spp. by using the polymerase chain reaction. Appl. Environ. Microbiol. 1992;58:2099–2101
  15. Leal-Klevezas DS, Martínez-Vázquez IO, López-Merino A, Martínez-Soriano JP. Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J. Clin. Microbiol. 1995;33:3087–3090
  16. Matar GM, Khneisser IA, Abdelnoor AM. Rapid laboratory confirmation of human brucellosis by PCR analysis of a target sequence on the 31-kilodalton Brucella antigen DNA. J. Clin. Microbiol. 1996;34:477–478
  17. Morata P, Queipo-Ortuño MI, de Dios Colmenero J. Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. J. Clin. Microbiol. 1998;36:2443–2446
  18. Morgan WJB, MacKinnon DJ. 2Brucellosis. In:  Laing JA editors. in Fertility and Infertility in Domestic Animals. 3rd ed.. London, UK: ELBS, Bailliere Tindall; 1979;p. 171–198
  19. Navarro E, Escribano J, Fernández J, Solera J. Comparison of three different PCR methods for detection of Brucella spp. in human blood samples. FEMS Immunol. Med. Microbiol. 2002;34:147–151
  20. O’Leary S, Sheahan M, Sweeney T. Brucella abortus detection by PCR assay in blood, milk and lymph tissue of serologically positive cows. Res. Vet. Sci. 2006;81:170–176
  21. OIE. Bovine brucellosis. in Manual of the Diagnostic Tests and Vaccines for Terrestrial Animals. Paris, France: Office International Des Epizooties; 2004;409-438
  22. Perry MB, Bundle DR. Antigenic relationships of the lipopolysaccharides of Escherichia hermannii strains with those of Escherichia coli O157:H7, Brucella melitensis, and Brucella abortus. Infect. Immun. 1990;58:1391–1395
  23. Price RE, Templeton JW, Smith R, Adams LG. Ability of mononuclear phagocytes from cattle naturally resistant or susceptible to brucellosis to control in vitro intracellular survival of Brucella abortus. Infect. Immun. 1990;58:879–886
  24. Quinn PJ, Carter ME, Markey B, Carter GR. Brucella species. In:  Quinn PJ,  Carter ME,  Markey BK,  Carter GR editor. Clinical Veterinary Microbiology. London, UK: Wolfe Publishing; 1994;p. 261–267
  25. Rijpens NP, Jannes G, Van Asbroeck M, Rossau R, Herman LM. Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S–23S rRNA spacer probes. Appl. Environ. Microbiol. 1996;62:1683–1688
  26. Romero C, Gamazo C, Pardo M, Lopez-Goni I. Specific detection of Brucella DNA by PCR. J. Clin. Microbiol. 1995;33:615–617
  27. Romero C, Lopez-Goni I. Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR. Appl. Environ. Microbiol. 1999;65:3735–3737
  28. Romero C, Pardo M, Grillo MJ, Diaz R, Blasco JM, Lopez-Goñi I. Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle. J. Clin. Microbiol. 1995;33:3198–3200
  29. Rossen L, Norskov P, Holmstrom K, Rasmussen OF. Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. Int. J. Food Microbiol. 1992;17:37–45
  30. Sreevatsan S, Bookout JB, Ringpis F, Perumaalla VS, Ficht TA, Adams LG, et al. A multiplex approach to molecular detection of Brucella abortus and/or Mycobacterium bovis infection in cattle. J. Clin. Microbiol. 2000;38:2602–2610
  31. Wilson IG. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 1997;63:3741–3751

PII: S0022-0302(08)71003-8

doi: 10.3168/jds.2008-1233

Journal of Dairy Science
Volume 91, Issue 10 , Pages 3779-3786 , October 2008