Journal of Dairy Science
Volume 91, Issue 8 , Pages 2938-2946 , August 2008

Microbial Community Analysis of Food-Spoilage Bacteria in Commercial Custard Creams Using Culture-Dependent and Independent Methods

  • K. Arakawa

      Affiliations

    • Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
  • ,
  • Y. Kawai

      Affiliations

    • Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
    • Corresponding Author InformationCorresponding author.
  • ,
  • H. Iioka

      Affiliations

    • Technology Development Laboratory, Tsukishima Foods Industry Co., Ltd., 3-17-9 Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan
  • ,
  • M. Tanioka

      Affiliations

    • Technology Development Laboratory, Tsukishima Foods Industry Co., Ltd., 3-17-9 Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan
  • ,
  • J. Nishimura

      Affiliations

    • Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
  • ,
  • H. Kitazawa

      Affiliations

    • Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
  • ,
  • K. Tsurumi

      Affiliations

    • Technology Development Laboratory, Tsukishima Foods Industry Co., Ltd., 3-17-9 Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan
  • ,
  • T. Saito

      Affiliations

    • Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan

Received 6 September 2007 ,Accepted 26 March 2008.

References 

  1. Amann RI, Ludwig W, Schleifer KH. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 1995;59:143–169
  2. Ampe F, ben Omar N, Moizan C, Wacher C, Guyot JP. Polyphasic study of the spatial distribution of microorganisms in Mexican pozol, a fermented maize dough, demonstrates the need for cultivation-independent methods to investigate traditional fermentations. Appl. Environ. Microbiol. 1999;65:5464–5473
  3. Ampe F, Sirvent A, Zakhia N. Dynamics of the microbial community responsible for traditional sour cassava starch fermentation studied by denaturing gradient gel electrophoresis and quantitative rRNA hybridization. Int. J. Food Microbiol. 2001;65:45–54
  4. ben Omar N, Ampe F. Microbial community dynamics during production of the Mexican fermented maize dough pozol. Appl. Environ. Microbiol. 2000;66:3664–3673
  5. Beukes EM, Bester BH, Mostert JF. The microbiology of South African traditional fermented milks. Int. J. Food Microbiol. 2001;63:189–197
  6. Blazevic DJ, Koepche MH, Matsen JM. Incidence and identification of Pseudomonas fluorescens and Pseudomonas putida in the clinical laboratory. Appl. Microbiol. 1973;25:107–110
  7. Boor KJ, Murphy SC. Microbiology of market milks. In:  Robinson RK editors. Dairy Microbiology Handbook. 3rd. edition. New York, NY: John Wiley and Sons Inc.; 2002;p. 91–122
  8. Bravo A, Sarabia S, Lopez L, Ontiveros H, Abarca C, Ortiz A, et al. Characterization of cry genes in a Mexican Bacillus thuringiensis strain collection. Appl. Environ. Microbiol. 1998;64:4965–4972
  9. Cenci-Goga BT, Crotti S, Costarelli S, Rondini C, Karama M, Bennett P. Detection of tet(M) gene from raw milk by rapid DNA extraction followed by a two-step PCR with nested primers. J. Food Prot. 2004;67:2833–2838
  10. Ceron J, Ortiz A, Quintero R, Guereca L, Bravo A. Specific PCR primers directed to identify cryI and cryIII genes within a Bacillus thuringiensis strain collection. Appl. Environ. Microbiol. 1995;61:3826–3831
  11. Chun J, Bae KS. Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences. Antonie Van Leeuwenhoek. 2000;78:123–127
  12. Cocolin L, Bisson L, Mills DA. Direct profiling of the yeast dynamics in wine fermentations. FEMS Microbiol. Lett. 2000;189:81–87
  13. Cocolin L, Manzano M, Cantoni C, Comi G. Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages. Appl. Environ. Microbiol. 2001;67:5113–5121
  14. Cotter PD, Hill C, Ross RP. Bacteriocins: Developing innate immunity for food. Nat. Rev. Microbiol. 2005;3:777–788
  15. Ellis RJ, Morgan P, Weightman AJ, Fry JC. Cultivation-dependent and -independent approaches for determining bacterial diversity in heavy-metal-contaminated soil. Appl. Environ. Microbiol. 2003;69:3223–3230
  16. Ercolini D. PCR-DGGE fingerprinting: Novel strategies for detection of microbes in food. J. Microbiol. Methods. 2004;56:297–314
  17. Fasoli S, Marzotto M, Rizzotti L, Rossi F, Dellaglio F, Torriani S. Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysis. Int. J. Food Microbiol. 2003;82:59–70
  18. Fontana C, Cocconcelli PS, Vignolo G. Monitoring the bacterial population dynamics during fermentation of artisanal Argentinean sausages. Int. J. Food Microbiol. 2005;103:131–142
  19. Fontana C, Vignolo G, Cocconcelli PS. PCR-DGGE analysis for the identification of microbial populations from Argentinean dry fermented sausages. J. Microbiol. Methods. 2005;63:254–263
  20. Harmon SM. New method for differentiating members of the Bacillus cereus group: Collaborative study. J. Assoc. Off. Anal. Chem. 1982;65:1134–1139
  21. Iwamoto T, Tani K, Nakamura K, suzuki Y, Kitagawa M, Eguchi M, et al. Monitoring impact of in situ biostimulation treatment on groundwater bacterial community by DGGE. FEMS Microbiol. Ecol. 2000;32:129–141
  22. Jensen MA, Straus N. Effect of PCR conditions on the formation of heteroduplex and single-stranded DNA products in the amplification of bacterial ribosomal DNA spacer regions. PCR Methods Appl. 1993;3:186–194
  23. Kanagawa T. Bias and artifacts in multitemplate polymerase chain reaction (PCR). J. Biosci. Bioeng. 2003;96:317–323
  24. Kawai Y, Saito T. Bacteriocins of lactic acid bacteria. In:  Shimazaki K,  Otani H editor. The Biol.-defensive Function of Dairy Foods. India: Research Signpost Trivandrum; 2002;p. 99–112
  25. Kroes I, Lepp PW, Relman DA. Bacterial diversity within the human subgingival crevice. Proc. Natl. Acad. Sci. USA. 1999;96:14547–14552
  26. Lane DJ. 16S/23S rRNA sequencing. In:  Stackebrandt ER,  Goodfellow M editor. Nucleic Acid Techniques in Bacterial Systematics. New York, NY: John Wiley and Sons Inc.; 1991;p. 115–175
  27. Lee JS, Heo GY, Lee JW, Oh YJ, Park JA, Park YH, et al. Analysis of kimchi microflora using denaturing gradient gel electrophoresis. Int. J. Food Microbiol. 2005;102:143–150
  28. Miambi E, Guyot JP, Ampe F. Identification, isolation and quantification of representative bacteria from fermented cassava dough using an integrated approach of culture-dependent and culture-independent methods. Int. J. Food Microbiol. 2003;82:111–120
  29. Muyzer G, de Waal EC, Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 1993;59:695–700
  30. Obodai M, Dodd CE. Characterization of dominant microbiota of a Ghanaian fermented milk product, nyarmie, by culture- and nonculture-based methods. J. Appl. Microbiol. 2006;100:1355–1363
  31. Randazzo CL, Torriani S, Akkermans AD, de Vos WM, Vaughan EE. Diversity, dynamics, and activity of bacterial communities during production of an artisanal Sicilian cheese as evaluated by 16S rRNA analysis. Appl. Environ. Microbiol. 2002;68:1882–1892
  32. Rantsiou K, Urso R, Iacumin L, Cantoni C, Cattaneo P, Comi G, et al. Culture-dependent and -independent methods to investigate the microbial ecology of Italian fermented sausages. Appl. Environ. Microbiol. 2005;71:1977–1986
  33. Ray B. Spoilage of specific food groups. In:  Ray B editors. Fundamental Food Microbiology. 3rd edition. Boca Raton, FL: CRC Press; 2004;p. 269–288
  34. Rosenquist H, Smidt L, Andersen SR, Jensen GB, Wilcks A. Occurrence and significance of Bacillus cereus and Bacillus thuringiensis in ready-to-eat food. FEMS Microbiol. Lett. 2005;250:129–136
  35. Schoeni JL, Wong AC. Bacillus cereus food poisoning and its toxins. J. Food Prot. 2005;68:636–648
  36. Torsvik V, Daae FL, Sandaa RA, Ovreas L. Novel techniques for analyzing microbial diversity in natural and perturbed environments. J. Biotechnol. 1998;17:53–62

PII: S0022-0302(08)71089-0

doi: 10.3168/jds.2007-0677

Journal of Dairy Science
Volume 91, Issue 8 , Pages 2938-2946 , August 2008