Binding of Mutagens to Exopolysaccharide Produced by Lactobacillus plantarum Mutant Strain 301102S

Agarose gel electrophoretic patterns of plasmid DNA sequences isolated from Lactobacillus plantarum strains 301102 and 301102S. Lane L=1-kb molecular weight ladder; lane 1=Lb. plantarum 301102; lane 2=Lb. plantarum 301102S; Chr = chromosomal DNA.

Microscopic observation of encapsulated cells stained with Indian ink. A = Lactobacillus plantarum 301102; B = mutant strain 301102S.

Binding ability against 3-amino-1,4-dimethyl-5H-pyrido indole (Trp-P-1) of strains 301102 and 301102S.

Binding of mutagens 3-amino-1,4-dimethyl-5H-pyrido indole (Trp-P-1), 2-amino-6-methyldipyrido imidazole (Glu-P-1), 2-amino-3,4-dimethylimidazo quinoline (MeIQ), and N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) to capsular cells of strain 301102S.

Effect of incubation time on the binding of 3-amino-1,4-dimethyl-5H-pyrido indole (Trp-P-1) to capsular cells of strain 301102S; incubation pH was 7.0.

Effect of pH on the binding of 3-amino-1,4-dimethyl-5 H-pyrido indole (Trp-P-1) to capsular cells of strain 301102S.

Effect of MgCl₂, SDS, and oxgall on the binding of 3-amino-1,4-dimethyl-5H-pyrido indole (Trp-P-1) to capsular cells of strain 301102S; incubation pH and time were 7.0 and 30min.