Journal of Dairy Science
Volume 91, Issue 5 , Pages 1751-1758 , May 2008

Production, Purification, and Characterization of a Potential Thermostable Galactosidase for Milk Lactose Hydrolysis from Bacillus stearothermophilus

Received 15 August 2007 ,Accepted 8 January 2008.

References 

  1. Bruins ME, Janssen AE, Boom RM. Thermozymes and their applications: A review of recent literature and patents. Appl. Biochem. Biotechnol. 2001;90:155–186
  2. Chen S. Studies on process engineering of galacto-oligosaccharides synthesis by beta-galactosidase from B. stearothermophilus. PhD thesis. Shanghai, China: East China University of Science and Technology; 2001;
  3. Chen W, Zhang H, Ding X. Study on properties of thermostable β-galactosidase and hydrolysis of lactose in milk. China Dairy Ind. 2002;30:15–18
  4. Chen W, Zhang H, Ge J, Ding X. High-level expression of thermostable galactosidase gene in Escherichia coli. Biotechnology (N. Y.). 2002;12:8–11
  5. Daabrowski S, Sobiewska G, Maciunska J, Synowiecki J, Kur J. Cloning, expression, and purification of the His(6)-tagged thermostable beta-galactosidase from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme. Protein Expr. Purif. 2000;19:107–112
  6. Demirjian DC, Moris-Varas F, Cassidy CS. Enzymes from extremophiles. Curr. Opin. Chem. Biol. 2001;5:144–151
  7. Ganeva V, Galutzov B, Eynard N. Electroinduced extraction of β-galactosidase from Kluyveromyces lactis. Appl. Microbiol. Biotechnol. 2001;56:411–413
  8. He Z, Peng Q, Chen J. Thermophiles Biology. Beijing, China: Science Press; 2000;
  9. Hirata H, Fukazawa T, Negoro S, Okada H. Structure of a β-galactosidase gene of Bacillus stearothermophilus. J. Bacteriol. 1986;166:722–727
  10. Hirata H, Negoro S, Okada H. High production of thermostable beta-galactosidase of Bacillus stearothermophilus in Bacillus subtilis. Appl. Environ. Microbiol. 1985;49:1547–1549
  11. Kang SK, Cho KK, Ahn JK, Bok JD, Kang SH, Woo JH, et al. Three forms of thermostable lactosehydrolase from Thermus sp. IB-21: Cloning, expression, and enzyme characterization. J. Biotechnol. 2005;116:337–346
  12. Lebbink JH, Kaper T, Bron P, van der Oost J, de Vos WM. Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus beta-glucosidase CelB by directed evolution. Biochemistry. 2000;39:3656–3665
  13. Onishi N, Tanaka T. Purification and properties of a novel thermostable galactooligosaccharide-producing beta-galactosidase from Sterigmatomyces elviae CBS8119. Appl. Environ. Microbiol. 1995;61:4026–4030
  14. Panesar PS, Panesar R, Singh RS, Kennedy JF, Kumar H. Microbial production, immobilization, and applications of beta-d -galactosidase. J. Chem. Technol. Biotechnol. 2006;81:530–543
  15. Prenosil JE, Stuker E, Bourne JR. Formation of oligosaccharides during enzymatic lactose hydrolysis. Part I: State of art. Biotechnol. Bioeng. 1987;30:1019–1025
  16. Robinson RK. 2nd ed.. Modern Dairy Technology. 1. London, UK: Chapman & Hall; 1994;
  17. Synowiecki J, Grzybowska B, Zdzieblo A. Sources, properties and suitability of new thermostable enzymes in food processing. Crit. Rev. Food Sci. 2006;46:197–205
  18. van Wely KHM, Swaving J, Freudl R, Driessen AJM. Translocation of proteins across the cell envelope of Gram-positive bacteria. FEMS Microbiol. Rev. 2001;25:437–454
  19. Wanarska M, Kur J, Pladzyk R, Turkiewicz M. Thermostable Pyrococcus woesei beta- d -galactosidase-high level expression, purification and biochemical properties. Acta Biochim. Pol. 2005;52:781–787
  20. Wei D, Chen S, Wang X, Yuan Q, Yu J. Properties of beta-galactosidase from Bacillus stearothermophilus. Microbiology. 2001;28:19–21
  21. Xia Y, Chen W, Fu X, Zhang H, Yang S, Ding X. Construction of an integrative food-grade expression system for Bacillus subtilis. Food. Res. Int. 2005;38:251–256
  22. Zeikus JG, Vieille C, Savchenko A. Thermozymes: Biotechnology and structure-function relationships. Extremophiles. 1998;2:179–183
  23. Zhao Y. The Principle of Biochemical Technology and Its Application. 3rd ed.. Beijing, China: Science Press; 2002;

PII: S0022-0302(08)71211-6

doi: 10.3168/jds.2007-617

Journal of Dairy Science
Volume 91, Issue 5 , Pages 1751-1758 , May 2008

Article Tools

* Image Usage & Resolution