Journal of Dairy Science
Volume 91, Issue 1 , Pages 1-10 , January 2008

Selective Separation of the Major Whey Proteins Using Ion Exchange Membranes

  • S. Goodall

      Affiliations

    • Department of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, United Kingdom
    • ,
  • A.S. Grandison

      Affiliations

    • Department of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, United Kingdom
    • Corresponding Author InformationCorresponding author.
    • ,
  • P.J. Jauregi

      Affiliations

    • Department of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, United Kingdom
    • ,
  • J. Price

      Affiliations

    • Volac International Limited, Felinfach, Lampeter, Ceredigion, Wales, SA48 8AG, United Kingdom

Received 23 July 2007 ,Accepted 5 September 2007.

References 

  1. Adisaputro IA, Wu Y-J, Etzel M. Strong cation and anion exchange membranes and beads for protein isolation from whey. J. Liquid Chromatogr. Related Technol. 1996;19:1437–1450
  2. Bhattacharjee S, Bhattacharjee C, Datta S. Studies on the fractionation of beta lactoglobulin from caesin whey using ultrafiltration and ion exchange membrane chromatography. J. Membr. Sci. 2006;275:141–150
  3. Casal E, Montilla A, Moreno FJ, Olano A, Corzo N. Use of chitosan for selective removal of beta lactoglobulin from whey. J. Dairy Sci. 2006;89:1384–1389
  4. Chiu CK, Etzel MK. Fractionation of lactoperoxidase and lactoferrin from bovine whey using a cation exchange membrane. J. Food Sci. 1997;62:996–1000
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  6. Drioli E, Romano M. Progress and new perspectives on integrated membrane operations for sustainable industrial growth. Ind. Eng. Chem. 2001;40:1277–1300
  7. Etzel M. Whey protein isolation and fractionation using ion exchangers. New York, NY: Marcel Dekker Inc; 1995;
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  11. Kanekanian ADA, Lewis MJ. Protein isolation using ion-exchangers. In:  Hudson BJF editors. Developments in Food Proteins. 4:London, UK: Elsevier Appl. Sci. Publ; 1986;p. 135–170
  12. Skudder P. Evaluation of a porous silica-based ion exchange medium for the production of protein fractions from rennet and acid whey. J. Dairy Res. 1985;52:167–181
  13. Smith P, Krohn R, Hermanson G, Mallia A, Gartner F, Provenzano M, et al. Measurement of protein using bicinchoninic acid. Anal. Biochem. 1985;150:76–85
  14. Splitt H, Mackenstedt I, Freitag R. Preparative membrane adsorber chromatography for the isolation of cow milk components. J. Chromatogr. A. 1996;729:87–97
  15. Turhan KN, Etzel MR. Whey protein isolate and alpha lactalbumin recovery from lactic acid whey using cation-exchange chromatography. J. Food Sci. 2004;69:66–70
  16. Ulber R, Plate K, Demmer T, Buchholz H, Scheper T. Downstream processing of bovine lactoferrin from sweet whey. Acta Biotechnol. 2001;21:27–34
  17. Weinbrenner WF, Etzel M. Competitive adsorption of alpha lactalbumin and bovine serum albumin to a sulfopropyl ion-exchange membrane. J. Chromatogr. A. 1994;662:414–419
  18. Zydney AL. Protein separations using membrane filtration: New opportunities for whey fractionation. Int. Dairy J. 1998;8:243–250

PII: S0022-0302(08)71431-0

doi: 10.3168/jds.2007-0539

Journal of Dairy Science
Volume 91, Issue 1 , Pages 1-10 , January 2008

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