Journal of Dairy Science
Volume 92, Issue 1 , Pages 79-86 , January 2009

Role of endogenous enzymes in proteolysis of sheep milk

Received 9 June 2008 ,Accepted 9 September 2008.

References 

  1. Albenzio M, Caroprese M, Marino R, Santillo A, Taibi L, Sevi A. Effects of somatic cell count and stage of lactation on the plasmin activity and cheese-making properties of ewe milk. J. Dairy Sci. 2004;87:533–542
  2. Andrews AT. Proteinases in normal bovine milk and their action on caseins. J. Dairy Res. 1983;50:45–55
  3. Baldi A, Savoini G, Cheli F, Fantuz F, Senatore E, Bertocchi L, et al. Changes in plasmin-plasminogen activator system in milk from Italian Friesian herds. Int. Dairy J. 1996;6:1045–1053
  4. Bieth J, Spiess B, Wermuth C. The synthesis and analytical use of a highly sensitive and convenient substrate of elastase. Biochem. Med. 1974;11:350–357
  5. Blakesley RW, Boezi JA. A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G250. Anal. Biochem. 1977;82:580–582
  6. Caroprese M, Marzano A, Schena L, Marino R, Santillo A, Albenzio M. Contribution of milk macrophages to proteolysis and to plasmin activity in ewe bulk milk. J. Dairy Sci. 2007;90:2767–2772
  7. Caroprese M, Marzano A, Schena L, Sevi A. Immunomagnetic procedure for positive selection of macrophages in ovine milk. J. Dairy Sci. 2008;91:1908–1912
  8. Clark S, Sherbon JW. Alpha s1-casein, milk composition and coagulation properties of goat milk. Small Rumin. Res. 2000;38:123–134
  9. Considine T, Healy A, Kelly AL, McSweeney PLH. Proteolytic specificity of elastase on bovine β-casein. Food Chem. 1999;66:463–470
  10. Considine T, Healy A, Kelly AL, McSweeney PLH. Proteolytic specificity of elastase on bovine αs1-casein. Food Chem. 2000;69:19–26
  11. Considine T, Healy A, Kelly AL, McSweeney PLH. Hydrolysis of bovine caseins by cathepsin B, a cysteine proteinase indigenous to milk. Int. Dairy J. 2004;14:117–124
  12. Cuccuru C, Moroni P, Zecconi A, Casu S, Caria A, Contini A. Milk differential cell counts in relation to total counts in Sardinian ewes. Small Rumin. Res. 1997;25:169–173
  13. Fox PF, Kelly AL. Indigenous enzymes in milk: Overview and historical aspects—Part 1. Int. Dairy J. 2006;16:500–516
  14. Hurley MJ, Larsen LB, Kelly AL, McSweeney PLH. The milk acid proteinase cathepsin D: A review. Int. Dairy J. 2000;10:673–681
  15. IDF. 1993. Determination of nitrogen content. Standard no. 20B. Int. Dairy Fed., Brussels, Belgium.
  16. IDF. 1995. Enumeration of somatic cells. Standard no. 148A. Int. Dairy Fed., Brussels, Belgium.
  17. Kelly AL, Fox PF. Indigenous enzymes in milk: A synopsis of future research requirements. Int. Dairy J. 2006;16:707–715
  18. Kelly AL, McSweeney PLH. Indigenous proteinases in milk. Adv. Dairy Chem. 2002;1:494–519
  19. Korycka-Dahl M, Ribadeau Dumas B, Chene N, Martal J. Plasmin activity in milk. J. Dairy Sci. 1983;66:704–711
  20. Larsen LB, Benfeldt C, Rasmussen LK, Petersen TE. Bovine milk procathepsin D and cathepsin D: Coagulation and milk protein degradation. J. Dairy Res. 1996;63:119–130
  21. Morgante M, Ranucci S, Paeselli M, Casoli C, Duranti E. Total and differential cell count in milk of primiparous Comisana ewes without clinical signs of mastitis. Small Rumin. Res. 1996;21:265–271
  22. Mulvihill DM, Fox PF. Proteolysis of αs1-casein by chymosin: Influence of pH and urea. J. Dairy Res. 1977;44:533–540
  23. O’Driscoll BM, Rattray FPP, McSweeney LH, Kelly AL. Protease activities in raw milk determined using a synthetic heptapeptide substrate. J. Food Sci. 1999;64:606–611
  24. Owen CA, Campbell EJ. The cell biology of leukocyte-mediated proteolysis. J. Leukoc. Biol. 1999;65:137–150
  25. Paape MJ, Bannerman DD, Zhao X, Lee JW. The bovine neutrophil: Structure and function in blood and in milk. Vet. Res. 2003;34:597–627
  26. Perlmann GE, Lorand L. Proteolytic enzymes. In:  Colowick SP,  Kaplan NO editor. Methods in enzymology. Vol. 19:New York, NY: Academic Press; 1970;p. 316–336
  27. Pillai SR, Kunze E, Sordillo LM, Jayarao BM. Application of differential inflammatory cell count as a tool to monitor udder health. J. Dairy Sci. 2001;84:1413–1420
  28. SAS Institute. 1999. SAS User's Guide: Statistics. Version 8.1 ed. SAS Inst. Inc., Cary, NC.
  29. Sevi A, Albenzio M, Marino R, Santillo A, Muscio A. Effects of lambing season and stage of lactation on ewe milk quality. Small Rumin. Res. 2004;51:251–259
  30. Sevi A, Albenzio M, Taibi L, Dantone D, Annicchioarico G, Massa S. Changes of somatic cell count through lactation and their effects on nutritional, renneting and bacteriological characteristics of ewes’ milk. Adv. Food Sci. 1999;21:122–127
  31. Smith R, Turk V. Cathepsin D: Rapid isolation by affinity chromatography on haemoglobin-agarose resin. Eur. J. Biochem. 1974;48:245–254
  32. Somers JM, O’Brien B, Meaney WJ, Kelly AL. Heterogeneity of proteolytic enzyme activities in milk samples of different somatic cell count. J. Dairy Res. 2003;70:45–50
  33. Verdi RJ, Barbano DM. Properties of proteases from milk somatic cells and blood leukocytes. J. Dairy Sci. 1991;74:2077–2081
  34. Walstra P, Jenness R, Badings HT. Dairy Chemistry and Physics. New York, NY: Wiley; 1984;

PII: S0022-0302(09)70311-X

doi: 10.3168/jds.2008-1439

Journal of Dairy Science
Volume 92, Issue 1 , Pages 79-86 , January 2009

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