Journal of Dairy Science
Volume 92, Issue 2 , Pages 444-457 , February 2009

Fate of lysostaphin in milk from individual cows through pasteurization and cheesemaking1

  • D.L. Van Hekken

      Affiliations

    • Dairy Processing and Products Research Unit, USDA, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038
    • Corresponding Author InformationCorresponding author.
    • ,
  • R.J. Wall

      Affiliations

    • Animal Biosciences and Biotechnology Lab, USDA, ARS, Beltsville Agricultural Research Center, Beltsville, MD 32611
    • ,
  • G.A. Somkuti

      Affiliations

    • Dairy Processing and Products Research Unit, USDA, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038
    • ,
  • M.A. Powell

      Affiliations

    • Animal Biosciences and Biotechnology Lab, USDA, ARS, Beltsville Agricultural Research Center, Beltsville, MD 32611
    • ,
  • M.H. Tunick

      Affiliations

    • Dairy Processing and Products Research Unit, USDA, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038
    • ,
  • P.M. Tomasula

      Affiliations

    • Dairy Processing and Products Research Unit, USDA, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038

Received 15 January 2008 ,Accepted 29 September 2008.

References 

  1. AOAC.. Official Methods of Analysis. 17th ed. Gaithersburg, MD: Assoc. Off. Anal. Chem. Int.; 2000;
  2. Browder HP, Zygmunt WA, Young JR, Tavormina PA. Lysostaphin: Enzymatic mode of action. Biochem. Biophys. Res. Commun. 1965;19:383–389
  3. Cavadini C, Hertel C, Hammes WP. Application of lysostaphin-producing lactobacilli to control staphylococcal food poisoning in meat products. J. Food Prot. 1998;61:419–424
  4. Code of Federal Regulations. 2003. Milk and cream. Title 21:290–291. http://www.cfsan.fda.gov/fct131.html Accessed May 24, 2007.
  5. Donovan DM, Dong S, Garrett W, Rousseau GM, Moineau S, Pritchard DG. Peptidoglycan hydrolase fusions maintain their parental specificities. Appl. Environ. Microbiol. 2006;72:2988–2996
  6. Douglas FW, Greenberg R, Farrell HM, Edmondson LF. Effects of ultra-high temperature pasteurization on milk proteins. J. Agric. Food Chem. 1981;29:11–15
  7. Fox PF, Cogan TM. Factors that affect the quality of cheese. In: 3rd ed.  Fox PF,  Mc Sweeney PLH,  Cogan TM,  Guinee TP editor. Cheese: Chemistry, Physics, and Microbiology. General Aspects. Vol.1:London, UK: Elsevier Ltd; 2004;p. 586–608
  8. Gaier W, Vogel RF, Hammes WP. Cloning and expression of the lysostaphin gene in Bacillus subtilis and Lactobacillus casei. Lett. Appl. Microbiol. 1992;14:72–76
  9. Harrison EF, Cropp CB. Therapeutic activity of lysostaphin in experimental staphylocooal infections. Can. J. Microbiol. 1967;13:93–97
  10. Heinrichs J, Jones C, Bailey K. Milk components: Understanding the causes and importance of milk fat and protein variation in your dairy herd. Dairy & Animal Science Fact Sheet. 1997;05-97:1e–8e
  11. Henderson JT, Chopko AL, van Wassenaar PD. Purification and primary structure of pediocin PA-1 produced by Pediococcus acidilactici PAC-1.0. Arch. Biochem. Biophys. 1992;295:5–12
  12. Kerr DE, Plaut K, Bramley AJ, Williamson CM, Lax AJ, Moore K, et al. Lysostaphin expression in mammary glands confers protection against staphylococcal infection in transgenic mice. Nat. Biotechnol. 2001;19:66–70
  13. Kerr DE, Wellnitz O. Mammary expression of new genes to combat mastitis. J. Anim. Sci. 2003;81(Suppl. 3):38–47
  14. Kokai-Kun JF, Walsh SM, Chanturiya T, Mond JJ. Lysostaphin cream eradicates Staphylococcus aureus nasal colonization in a cotton rat model. Antimicrob. Agents Chemother. 2003;47:1589–1597
  15. Kosikowski FV, Mistry VV. Cheese and fermented milk foods. In: 3rd ed.  Kosikowski FV editors. Procedures and Analysis. Vol. 2:Westport, CT: LLC; 1997;p. 90–96
  16. Langlois BE, Harmon RJ, Akers K. Use of lysostaphin and bacitracin susceptibility for routine presumptive identification of staphylococci of bovine origin. J. Food Prot. 1988;51:24–28
  17. Maga EA, Anderson GB, Cullor JS, Smith W, Murray JD. Antimicrobial properties of human lysozyme transgenic mouse milk. J. Food Prot. 1998;61:52–56
  18. Maga EA, Anderson GB, Murray JD. The effect of mammary gland expression of human lysozyme on the properties of milk from transgenic mice. J. Dairy Sci. 1995;78:2645–2652
  19. Maga EA, Shoemaker CF, Rowe JD, BonDurant RH, Anderson GB, Murray JD. Production and processing of milk from transgenic goats expressing human lysozyme in the mammary gland. J. Dairy Sci. 2006;89:518–524
  20. Mc Kellar RC, Liou S, Modler HW. Predictive modeling of lactoperoxidase and γ-glutamyl transpeptidase inactivation in a high-temperature short-time pasteurizer. Int. Dairy J. 1996;6:295–301
  21. Metcalf RH, Deibel RH. Staphylococcus aureus response to lysostaphin in some fermented foods. Appl. Microbiol. 1969;17:63–67
  22. Norman HD, Lawlor TJ, Wright JR, Powell RL. Performance of Holstein clones in the United States. J. Dairy Sci. 2004;87:729–738
  23. Oldham ER, Daley MJ. Lysostaphin: Use of a recombinant bactericidal enzyme as a mastitis therapeutic. J. Dairy Sci. 1991;74:4175–4182
  24. Quickel KE, Selden R, Caldwell JR, Nora NF, Schaffner W. Efficacy and safety of topical lysostaphin treatment of persistent nasal carriage of Staphylococcus aureus. Appl. Environ. Microbiol. 1971;22:446–450
  25. Rainard P. Tackling mastitis in dairy cows. Nat. Biotechnol. 2005;23:430–432
  26. Rocco MR. Fluorometric determination of alkaline phosphatase in fluid dairy products: Collaborative study. Assoc. Off. Anal. Chem. 1990;73:842–849
  27. SAS Institute. SAS User's Guide: Statistics. Cary, NC.: SAS Inst. Inc.; 2001;Version 8.2
  28. Schindler CA, Schuhardt VT. Purification and properties of lysostaphin - A lytic agent for Staphylococcus aureus. Biochim. Biophys. Acta. 1965;97:242–250
  29. Sutra L, Poutrel B. Virulence factors involved in the pathogenesis of bovine intramammary infections due to Staphylococcus aureus. J. Med. Microbiol. 1994;40:79–89
  30. Tian XC, Kubota C, Sakashita K, Izaike Y, Okano R, Tabara N, et al. Meat and milk compositions of bovine clones. Proc. Natl. Acad. Sci. USA. 2005;102:6261–6266
  31. Tunick MH, Malin EL, Smith PW, Holsinger VH. Effect of skim milk homogenization on proteolysis and rheology of Mozzarella cheese. Int. Dairy J. 1995;5:483–491
  32. Van Hekken DL, Tunick MH, Tomasula PT, Molina Corral FJ, Gardea AA. Mexican Queso Chihuahua: Rheology of fresh cheese. Int. J. Dairy Technol. 2007;60:5–12
  33. Wall RJ, Powell AM, Paape MJ, Kerr DE, Bannerman DD, Pursel VG, et al. Genetically enhanced cows resist intramammary Staphylococcus aureus infection. Nat. Biotechnol. 2005;23:445–451

PII: S0022-0302(09)70348-0

doi: 10.3168/jds.2008-1019

Journal of Dairy Science
Volume 92, Issue 2 , Pages 444-457 , February 2009

Article Tools

* Image Usage & Resolution