Evaluation of the allergenicity of goat milk, cow milk, and their lactosera in a guinea pig model

Sodium dodecyl sulfate-PAGE pattern of goat and cow milk and their respective lactosera. Lane 1 = prestained molecular weight marker (Bio-Rad, Hercules, CA); lane 2 = goat β-casein marker isolated by Technical Services (Granada University, Spain); lane 3 = rabbit IgG marker (Serotec, Oxford, UK); lane 4 = goat milk (GM); lane 5 = cow milk (CM); lane 6 = goat milk lactoserum (GML); and lane 7 = cow milk lactoserum (CML).

Mean (±SE) of the optical density (OD) values of specific IgG1 in animals sensitized by oral route to goat milk (GM, ■), cow milk (CM, □), goat milk lactoserum (GML, •), and cow milk lactoserum (CML, ○).

Mean (±SE) of the optical density (OD) values of specific IgG(Fc) in animals sensitized by oral route to goat milk (GM, ■), cow milk (CM, □), goat milk lactoserum (GML, •), and cow milk lactoserum (CML, ○).

Western blot developed against the sera of animals used for the systemic anaphylaxis assay, sensitized to goat milk (GM) and cow milk (CM). Lane 1 = prestained molecular weight marker (Bio-Rad, Hercules, CA); lane 2 = β-casein from GM, isolated by Technical Services (Granada University, Spain) and incubated with antibodies against GM; lane 3 = GM proteins subjected to electrophoresis and incubated with antibodies against GM; lane 4 = CM proteins subjected to electrophoresis and incubated with antibodies against CM; lane 5 = goat milk lactoserum (GML) proteins subjected to electrophoresis and incubated with antibodies against GML; and lane 6 = cow milk lactoserum (CML) proteins subjected to electrophoresis and incubated with antibodies against CML.

Western blot developed against the sera of animals used for the systemic anaphylaxis assay, sensitized to goat milk (GM) and cow milk (CM). Lane 1 = prestained molecular weight marker (Bio-Rad, Hercules, CA); lane 2 = GM proteins subjected to electrophoresis and incubated with antibodies against GM; lane 3 = CM proteins subjected to electrophoresis and incubated with antibodies against GM; lane 4 = CM proteins subjected to electrophoresis and incubated with antibodies against CM; and lane 5 = GM proteins subjected to electrophoresis and incubated with antibodies against CM.