Journal of Dairy Science
Volume 92, Issue 4 , Pages 1354-1360 , April 2009

Rapid detection of bovine milk in yak milk using a polymerase chain reaction technique

  • W.L. Bai

      Affiliations

    • College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161, China
    • College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China
    • ,
  • R.H. Yin

      Affiliations

    • College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161, China
    • ,
  • S.J. Zhao

      Affiliations

    • Animal Science Research Academy of Sichuan Province, Chengdu 610066, China
    • ,
  • Q.L. Dou

      Affiliations

    • College of Agricultural and Animal Husbandry, Qinghai University, Xining 810016, China
    • ,
  • J.C. Yang

      Affiliations

    • College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161, China
    • ,
  • W.Q. Jiang

      Affiliations

    • College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161, China
    • ,
  • Z.H. Zhao

      Affiliations

    • College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China
    • ,
  • G.B. Luo

      Affiliations

    • College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161, China
    • Corresponding Author InformationCorresponding author.

Received 17 September 2008 ,Accepted 18 November 2008.

References 

  1. Addeo F, Moio L, Chianese L, Stingo C, Resmini P, Berner I, et al. Use of plasmin to increase the sensitivity of the detection of bovine milk in ovine and/or caprine cheese by gel isoelectric focusing of γ2-caseins. Milchwissenschaft. 1990;45:708–711
  2. Amills M, Francino O, Jansa M, Sánchez A. Isolation of genomic DNA from milk samples by using Chelex resin. J. Dairy Res. 1997;64:231–238
  3. Bania J, Ugorski M, Polanowski A, Adamczyk E. Application of polymerase chain reaction for detection of goats’ milk adulteration by milk of cow. J. Dairy Res. 2001;68:333–336
  4. Bottero MT, Civera T, Anastasio A, Turi RM, Rosati S. Identification of cow's milk in “buffalo” cheese by duplex polymerase chain reaction. J. Food Prot. 2002;65:362–366
  5. Bottero MT, Civera T, Nucera D, Rosati S, Sacchi P, Turi RM. A multiplex polymerase chain reaction for the identification of cows’, goats’, and sheep's milk in dairy products. Int. Dairy J. 2003;13:277–282
  6. Bramanti E, Sortino C, Raspi G, Synovec RE. Separation and determination of denatured caseins by hydrophobic interaction chromatography. Part II. Method validation and applications. Analyst (Lond.). 2001;126:995–1000
  7. Branciari R, Nijman IJ, Plas ME, Di Antonio E, Lenstra JA. Species origin of milk in Italian Mozzarella and Greek feta cheese. J. Food Prot. 2000;63:408–411
  8. Chianese L, Laezza P, Smaldone LA, Stingo C, Del Giovine L, Addeo F. Evaluation of bovine milk in the buffalo Mozzarella cheese by two-dimensional electrophoresis. Sci. Tecn. Latt. Cas. 1990;41:315–326
  9. Colgan S, O’Brien L, Maher M, Shilton N, McDonnell K, Ward. S. Development of a DNA-based assay for species identification in meat and bone meal. Fleischwirtschaft. 2001;34:409–414
  10. Dalmasso A, Fontanella E, Piatti P, Civera T, Rosati S, Bottero MT. A multiplex PCR assay for the identification of animal species in feedstuffs. Mol. Cell. Probes. 2004;18:81–87
  11. De Noni I, Tirelli A, Masotti F. Detection of cows’ milk in non-bovine cheese by HPLC of whey protein: Application to goat milk cheese. Sci. Tecn. Latt. Cas. 1996;47:7–17
  12. Fan BL, Li N, Hu XX, Wu CX. Cloning, sequencing and polymorphism analyzing of the exon 4 of the kappa-casein gene in yak. Prog. Nat. Sci. 2000;10:769–773
  13. Hall TA. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 1999;41:95–98
  14. Haza AI, Morales P, Martín R, García T, Aguita G, González I, et al. Use of a monoclonal antibody and two enzyme-linked inmunosorbent assay formats for detection and quantification of the substitution of caprine milk for ovine milk. J. Food Prot. 1997;60:973–977
  15. Hurley IP, Coleman RC, Ireland HE, Williams JHH. Measurement of bovine IgG by indirect competitive ELISA as a means of detecting milk adulteration. J. Dairy Sci. 2004;87:543–549
  16. Hurley IP, Ireland HE, Coleman RC, Williams JHH. Application of immunological methods for the detection of species adulteration in dairy products. Int. J. Food Sci. Technol. 2004;39:873–878
  17. Kudo T, Sato S, Sutou S. Sexing of bovine embryos with male-specific repetitive DNA by polymerase chain reaction: Cloning and characterization of bovine male-specific repetitive DNA. J. Reprod. Dev. 1993;39:55–63
  18. Lipkin E, Shalom A, Khatib H, Soller M, Friedmann A. Milk as a source of deoxyribonucleic acid and as a substrate for the polymerase chain reaction. J. Dairy Sci. 1993;76:2025–2032
  19. López-Calleja I, González I, Fajardo V, Martín I, Hernández PE, García T, et al. Application of polymerase chain reaction to detect adulteration of sheep's milk with goats’ milk. J. Dairy Sci. 2005;88:3115–3120
  20. López-Calleja I, González I, Fajardo V, Rodríguez MA, Hernandez PE, García T, et al. Rapid detection of cows’ milk in sheeps’ and goats’ milk by a species-specific polymerase chain reaction technique. J. Dairy Sci. 2004;87:2839–2845
  21. Mafra I, Ferreira IMPLVO, Faria MA, Oliveira BPP. A novel approach to the quantification of bovine milk in ovine cheeses using a duplex polymerase chain reaction method. J. Agric. Food Chem. 2004;52:4943–4947
  22. Maudet C, Taberlet P. Detection of cows’ milk in goats’ cheeses inferred from mitochondrial DNA polymorphism. J. Dairy Res. 2001;68:229–235
  23. Mayer HK, Heidler D, Rockenbauer C. Determination of the percentages of cows’, ewes’ and goats’ milk in cheese by isoelectric focusing and cation-exchange HPLC of γ- and para-κ- caseins. Int. Dairy J. 1997;7:619–628
  24. Moatsou G, Hatzinaki A, Psathas G, Anifantakis E. Detection of caprine casein in ovine Halloumi cheese. Int. Dairy J. 2004;3:219–226
  25. Moio L, Sasso ML, Chianese L, Addeo F. Rapid detection of bovine milk in ovine, caprine and water buffalo milk or cheese by gel isoelectric focusing on phastsystem. Ital. J. Food Sci. 1990;3:185–190
  26. Molina E, Martín-Álvarez PJ, Ramos M. Analysis of cows’, ewes’ and goats’ milk mixtures by capillary electrophoresis: Quantification by multivariate regression analysis. Int. Dairy. J. 1999;9:99–105
  27. Partis L, Croan D, Guo Z, Clark R, Coldham T, Murby J. Evaluation of a DNA finger printing method for determining the species origin of meats. Meat Sci. 2000;54:369–376
  28. Rea S, Chikuni K, Branciari R, Sukasi Sangamayya R, Ranucci D, Avellini P. Use of duplex polymerase chain reaction (duplex PCR) technique to identify bovine and water buffalo milk used in making Mozzarella cheese. J. Dairy Res. 2001;68:689–698
  29. Reale S, Campanella A, Merigioli A, Pilla F. A novel method for species identification in milk and milk-based products. J. Dairy Res. 2008;75:107–112
  30. Recio I, García-Risco MR, Amigo L, Molina E, Ramos M, Martín-Álvarez PJ. Detection of milk mixtures in Halloumi cheese. J. Dairy Sci. 2004;87:1595–1600
  31. Richter W, Krause I, Graf C, Sperrer I, Schwarzer C, Klostermeyer H. An indirect competitive ELISA for detection of cows’ milk and caseinate in goats’ and ewes’ milk and cheese using polyclonal antibodies against bovine γ-caseins. Z. Lebensm. Unters. Forsch. 1997;204:21–26
  32. Rodríguez MA, García T, González I, Asensio L, Mayoral B, López-Calleja I, et al. Development of a polymerase chain reaction assay for species identification of goose and mule duck in foie gras products. Meat Sci. 2002;65:1257–1263
  33. Sasaki, M. 1994. Yak: Hardy multi-purpose animal of Asia highland. Pages 1–5 in Proc. 1st Int. Congr. Yak, Lanzhou, China. Supplement of J. Gansu Agric. Univ., Lanzhou, China.
  34. Tartaglia M, Saulle E, Pestalozza S, Morelli L, Antonucci G, Battaglia PA. Detection of bovine mitochondrial DNA in ruminant feeds: A molecular approach to test for the presence of bovine-derived materials. J. Food Prot. 1998;61:513–518
  35. Torre M, Cohen ME, Corzo N, Rodrı’guez MA, Diez-Masa. JC. Perfusion liquid chromatography of whey proteins. J. Chromatogr. A. 1996;729:99–111
  36. Unseld M, Beyermann D, Brandt P, Hiesel R. Identification of the species origin of highly processed meat products by mitochondrial DNA sequences. PCR Methods Appl. 1995;4:241–243
  37. Urbanke W, Luf W, Brandl E. Einsatz der HPLC bei der verfalschungskontrolle von milch und michprodukten verschiedener species. Z. Lebensm. Unters. Forsch. 1992;195:137–142
  38. Wang, Y. S., and S. X. Zou. 1995. Protein in milk. Pages 1–48 in Biochemistry of Milk. Jilin Univ. Press, Changchun, China.
  39. Wiener, G., J. L. Han, and R. J. Long. 2003. Origins, domestication and distribution of yak, and production characteristics of yak. Pages 2, 136–137 in The Yak. 2nd ed. Regional Office for Asia and the Pacific of the Food and Agriculture Organization of the United Nations, Bangkok, Thailand.

PII: S0022-0302(09)70446-1

doi: 10.3168/jds.2008-1727

Journal of Dairy Science
Volume 92, Issue 4 , Pages 1354-1360 , April 2009

Article Tools

* Image Usage & Resolution