Journal of Dairy Science
Volume 92, Issue 6 , Pages 2382-2388 , June 2009

Rapid detection of Salmonella in milk by combined immunomagnetic separation-polymerase chain reaction assay

  • B. Mercanoglu Taban

      Affiliations

    • Gazi University, Vocational School of Health Services, Golbasi, Ankara, Turkey
    • Corresponding Author InformationCorresponding author.
    • ,
  • U. Ben

      Affiliations

    • Hacettepe University, Faculty of Engineering, Food Engineering Department, Beytepe, Ankara, Turkey
    • ,
  • S.A. Aytac

      Affiliations

    • Hacettepe University, Faculty of Engineering, Food Engineering Department, Beytepe, Ankara, Turkey

Received 8 July 2008 ,Accepted 10 February 2009.

References 

  1. CDC (Centers for Disease Control). 2003. Multistate outbreak of Salmonella serotype Typhimurium infections associated with drinking unpasteurized milk–Illinois, Indiana, Ohio, and Tennessee, 2002—2003. MMWR Morb. Mortal. Wkly. Rep. 52:613–615.
  2. CDC (Centers for Disease Control). 2007. Salmonella typhimurium infection associated with raw milk and cheese consumption–Pennsylvania, 2007. MMWR Morb. Mortal. Wkly. Rep. 56:1161-1164.
  3. Cody SH, Abbott SL, Marfin AA, Schulz B, Wagner P, Robbins K, et al. Two outbreaks of multidrug-resistant Salmonella serotype typhimurium DT104 infections linked to raw-milk cheese in Northern California. JAMA. 1999;281:1805–1810
  4. Garcia-del Portillo F. Molecular and cellular biology of Salmonella pathogenesis. In: Microbial Foodborne Diseases: Mechanisms of Pathogenesis and Toxin Synthesis. Lancaster, PA: Technomic Publishing Company; 2000;p. 1–51
  5. Haeghebaert S, Sulem P, Deroudille L, Vanneroy-Adenot E, Bagnis O, Bouvet P, et al. Two outbreaks of Salmonella enteritidis phage type 8 linked to the consumption of Cantal cheese made with raw milk, France, 2001. Eur. Surv. 2003;8:151–156
  6. Hsih HY, Tsen H-Y. Combination of immunomagnetic separation and polymerase chain reaction for the simultaneous detection of Listeria monocytogenes and Salmonella spp. in food samples. J. Food Prot. 2001;64:1744–1750
  7. ISO (International Organization for Standardization). Microbiology of food and animal feeding stuffs—Horizontal method for the detection of Salmonella spp. ISO. Switzerland: Geneva; 2002;
  8. Jeníková G, Pazlarová J, Demnerová K. Detection of Salmonella in food samples by the combination of immunomagnetic separation and PCR assay. Int. Microbiol. 2000;3:225–229
  9. Löfström C, Knutsson R, Axelsson CE, Rådström P. Rapid and specific detection of Salmonella spp. in animal feed samples by PCR after culture enrichment. Appl. Environ. Microb. 2004;70:69–75
  10. Mandrell RE, Wachtel MR. Novel detection techniques for human pathogens that contaminate poultry. Curr. Opin. Biotechnol. 1999;10:273–278
  11. Mansfield LP, Forsythe SJ. Collaborative ring-trial of Dynabeads® anti-Salmonella for immunomagnetic separation of stressed Salmonella cells from herbs and spices. Int. J. Food Microbiol. 1996;29:41–47
  12. Mazurek J, Salehi E, Propes D, Holt J, Bannerman T, Nicholson LM, et al. A multistate outbreak of Salmonella enterica serotype typhimurium infection linked to raw milk consumption–Ohio, 2003. J. Food Prot. 2004;67:2165–2170
  13. Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, et al. Food-related illness and death in the United States. Emerg. Infect. Dis. 1999;5:607–625
  14. Mercanoglu B, Aytac SA. Immunomagnetic separation and a cultural reference method for detection of Salmonella spp. in foods. Arch. Lebensmittelhyg. 2002;53:43–45
  15. Mercanoglu B, Griffiths MW. Combination of immunomagnetic separation with real-time PCR for rapid detection of Salmonella in milk, ground beef, and alfalfa sprouts. J. Food Prot. 2005;68:557–561
  16. Oliveira SD, Santos LR, Schuch DMT, Silva AB, Salle CTP, Canal CW. Detection and identification of salmonellas from poultry-related samples by PCR. Vet. Microbiol. 2002;87:25–35
  17. Rahn K, de Grandis SA, Clarke RC, McEwen SA, Galan JE, Ginocchio C, et al. Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method for detection of Salmonella. Mol. Cell. Probes. 1992;6:271–279
  18. Rijpens N, Herman L, Vereecken F, Jannes G, Smedt JD, Zutter LD. Rapid detection of stressed Salmonella spp. in dairy and egg products using immunomagnetic separation and PCR. Int. J. Food Microbiol. 1999;46:37–44
  19. Skjerve E, Olsvik O. Immunomagnetic separation of Salmonella from foods. Int. J. Food Microbiol. 1991;14:11–18
  20. Soumet C, Blivet D, Ermel G, Colin P, Salvat G. An immunoconcentration-PCR assay to detect Salmonella in the environment of poultry houses. Int. J. Food Microbiol. 1999;48:221–224
  21. Španová A, Rittich B, Horák D, Lenfeld J, Prodělalová J, Sučiková J, et al. Immunomagnetic separation and detection of Salmonella cells using newly designed carriers. J. Chromatogr. A. 2003;1009:215–221
  22. Stevens KA, Jaykus L-A. Direct detection of bacterial pathogens in representative dairy products using a combined bacterial concentration-PCR approach. J. Appl. Microbiol. 2004;97:1115–1122
  23. Suslow TV, Wu J, Fett WF, Harris LJ. Detection and elimination of Salmonella Mbandaka from naturally contaminated alfalfa seed by treatment with heat or calcium hypochlorite. J. Food Prot. 2002;65:452–458
  24. Widjojoatmodjo MN, Fluit AC, Torensma R, Keller BHI, Verhoef J. Evaluation of the magnetic immuno PCR assay for rapid detection of Salmonella. Eur. J. Clin. Microbiol. 1991;10(Suppl. 11):935–938

PII: S0022-0302(09)70553-3

doi: 10.3168/jds.2008-1537

Journal of Dairy Science
Volume 92, Issue 6 , Pages 2382-2388 , June 2009

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