Variations in bovine milk oligosaccharides during early and middle lactation stages analyzed by high-performance liquid chromatography-chip/mass spectrometry

High-performance liquid chromatography-chip/time-of-flight-MS chromatograms of bovine milk oligosaccharides of colostrum (d 1), d 6, and d 120 milk from Holstein cow 1950. RT=retention time.

Extracted ion chromatograms of selected ions (A) m/z 636.2346 and (B) m/z 710.2714 from colostrum (d 1) of Holstein cow 1950 with mass spectra inset. RT=retention time.

High-performance liquid chromatography-chip/time-of-flight chromatograms of bovine colostrum oligosaccharides from Jersey cows (3 runs overlapped). RT=retention time.

High-performance liquid chromatography-chip/time-of-flight chromatograms of bovine colostrum oligosaccharides from Holstein cow 1950, cow 1938, and cow 2069. RT=retention time.

Changes in the total oligosaccharides (OS), total anionic OS, and N-glycolylneuraminic acid (NeuGc)-containing OS only content of bovine milk at different stages of lactation (colostrum, d 6, and d 120 representing colostrum, transitional, and mature milk, respectively). Values (mean ± SD) are n=9 for colostrum and d 6, and n=4 for d 120 milk samples. RT=retention time.

Extracted ion chromatograms of ion m/z 636.2346 from 4 Jersey cows at different lactation points. RT=retention time.

Changes in the total oligosaccharide intensity (A) and sialylation percentage (B) of Jersey cows (cows 630, 632, 636, and 643) at different stages of lactation (colostrum, d 6, and d 120 representing colostrum, transitional, and mature milk, respectively).