Nisin and lacticin 481 coproduction by Lactococcus lactis strains isolated from raw ewes’ milk

SmaI pulsotypes and dendrogram obtained with 13 isolates of bacteriocin-producing lactococci from raw ewes’ milk.

Amino acid sequence of nisin from Lactococcus lactis ssp. lactis INIA Z108, deduced from the DNA sequence and compared with the sequences of nisin A, nisin Z, nisin Q, and nisin F. Differences in AA are indicated by underlined letters.

Separation of bacteriocins from culture supernatants by SDS-PAGE and overlaid with de Man, Rogosa, Sharpe agar (Difco Laboratories, Detroit, MI) with Lactococcus lactis HP as an indicator microorganism: 1) negative control, 2) L. lactis INIA Z103, 3) L. lactis INIA Z101, 4) L. lactis INIA Z108, 5) L. lactis INIA 626 + L. lactis INIA 639, 6) L. lactis INIA 626, 7) L. lactis INIA 639, 8) supernatant from L. lactis INIA Z103 after silver staining.