Journal of Dairy Science
Volume 93, Issue 3 , Pages 841-848 , March 2010

Characterization of wine rennet and its kinetics by gel electrophoresis

Received 8 May 2009 ,Accepted 27 November 2009.

References 

  1. Aikawa J, Yamashita T, Nishiyama M, Horinouchi S, Beppu T. Effects of glycosylation on the secretion and enzyme activity of Mucor rennin, an aspartic proteinase of Mucor pusillus, produced by recombinant yeast. J. Biol. Chem. 1990;265:13955–13959
  2. Andrews AT. Proteinases in normal bovine milk and their action on caseins. J. Dairy Res. 1983;50:45–55
  3. Athaudaa SB, Takahashia K. Cleavage specificities of aspartic proteinases towards oxidized insulin B chain at different pH values. Protein Pept. Lett. 2002;9:289–294
  4. Azarnia S, Robert N, Lee B. Biotechnological methods to accelerate cheddar cheese ripening. Crit. Rev. Biotechnol. 2006;26:121–143
  5. Barkholt V. Amino acid sequence of endothiapepsin. Complete primary structure of the aspartic protease from Endothia parasitica. Eur. J. Biochem. 1987;167:327–338
  6. Carles C, Martin P. Kinetic study of the action of bovine chymosin and pepsin on bovine κ-casein. Arch. Biochem. Biophys. 1985;242:411–416
  7. Carles C, Ribadeau Dumas B. Kinetics of the action of chymosin (rennin) on a peptide bond of bovine αs1-casein. Comparison of the behavior of this substrate with that of β- and κ-caseins. FEBS Lett. 1985;185:282–286
  8. Costa J, Ashford DA, Nimtz M, Bento I, Frazao C, Estevez CL, et al. The glycosylation of the aspartic proteinases from barley (Hordeum vulgare L.) and cardoon (Cynara cardunculus L.). Eur. J. Biochem. 1997;243:695–700
  9. Gaiaschi A, Beretta B, Poiesi C, Conti A, Giuffrida MG, Galli CL, et al. Proteolysis of αS-casein as a marker of Grana Padano cheese ripening. J. Dairy Sci. 2000;83:2733–2739
  10. Gaiaschi A, Beretta B, Poiesi C, Conti A, Giuffrida MG, Galli CL, et al. Proteolysis of β-casein as a marker of Grana Padano cheese ripening. J. Dairy Sci. 2001;84:60–65
  11. Garg SK, Johri BN. Rennet: Current trends and future research. Food Rev. Int. 1994;10:313–355
  12. Hidaka M, Sasaki K, Uozumi T, Beppu T. Cloning and structural analysis of the calf prochymosin gene. Gene. 1986;43:197–203
  13. Hill RD, Lahav E, Givol D. A rennin-sensitive bond in alpha-S1 B-casein. J. Dairy Res. 1974;41:147–153
  14. Jiang T, Chen LJ, Xue L, Chen LS. Study on milk-clotting mechanism of rennet-like enzyme from glutinous rice wine: Proteolytic property and the cleavage site on κ-casein. J. Dairy Sci. 2007;90:3126–3133
  15. Jiang TM, Xue L, Zhou WM, Chen LJ. Purification and preliminary discussion on coagulation mechanism of rennet from glutinous rice wine. Food Ferment. Ind. 2006;32:24–27
  16. Liu ZM, Liu H, Lao CX. Studies on the screening of rennet producing strain and its culture conditions. Food Ferment. Ind. 2001;27:8–11
  17. Machalinski C, Pirpignani ML, Marino C, Mantegazza A, de Jiménez Bonino MB. Structural aspects of the Mucor bacilliformis proteinase, a new member of the aspartyl-proteinase family. J. Biotechnol. 2006;123:443–452
  18. Poza M, Prieto-Alcedo M, Sieiro C, Villa TG. Cloning and expression of clt genes encoding milk-clotting protease from Myxococcus xanthus 422. Appl. Environ. Microbiol. 2004;70:6337–6341
  19. Rickert WS, McBride-Warren PA. Structural and functional determinants of Mucor miehei protease. Biochim. Biophys. Acta. 1974;336:437–444
  20. Shammet KM, Brown RJ, McMahon DJ. Proteolytic activity of some milk-clotting enzymes on κ-casein. J. Dairy Sci. 1992;75:1373–1379
  21. Takahashi K. Determination of the amino acid sequences of the two major isozymes of rhizopuspepsin. J. Biochem. 1988;103:162–167
  22. Teng, G. X. 2005. Characteristics of Rhizopus milk-clotting enzyme in jiu-yao and studies on texture of kou wan lao. PhD thesis. China Agric. Univ., Beijing.
  23. Visser S, Rollema HS. Quantification of chymosin action on nonlabeled kappa-casein-related peptide substrates by ultraviolet spectrophotometry: description of kinetics by the analysis of progress curves. Anal. Biochem. 1986;153:235–241
  24. Visser S, Van Rooijen PJ, Slangen CJ. Peptide substrates for chymosin (rennin) Isolation and substrate behaviour of two tryptic fragments of bovine κ-casein. Eur. J. Biochem. 1980;108:415–421
  25. Vreeman HJ, Visser S, Slangen CJ, Van Riel JA. Characterization of bovine κ-casein fractions and the kinetics of chymosin-induced macropeptiede release from carbohydrate-free and carbohydrate-containing fractins determined by high-performance gel-permeation chromatography. Biochem. J. 1986;240:87–97
  26. Xue L, Chen LJ, Jiang TM, Zhou WM. Study on properties of chymosin purified from glutinous rice wine. Food Sci. 2008;29:259–262

PII: S0022-0302(10)00050-0

doi: 10.3168/jds.2009-2364

Journal of Dairy Science
Volume 93, Issue 3 , Pages 841-848 , March 2010

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