Self-assembled β-lactoglobulin–conjugated linoleic acid complex for colon cancer-targeted substance

Spectrofluorometric analysis of the binding of cis-9, trans-11 conjugated linoleic acid (CLA) to bovine β-LG at pH 7.0 and 25°C. The symbols represent experimental points of triplicate titration runs.

Particle size distribution of β-LG–conjugated linoleic acid complex at pH 7.0 and 25°C. Color version available in the online PDF.

Zeta potential of β-LG–conjugated linoleic acid complex as a function of pH in gastrointestinal conditions.

The SDS-PAGE patterns obtained from β-LG–conjugated linoleic acid (CLA) complex solution as a function of digestive enzymes. A) The continuous phase of β-LG-CLA complex treated by pepsin in 2h; B) the continuous phase of β-LG-CLA complex treated by trypsin in 2h. Bands a, b, and c are 3 major bands that appeared in the SDS-PAGE during gastrointestinal digestion. The molecular weight of a is about 36.8 kDa, b is about 18.4 kDa, and c is about 10 to 16 kDa. M=molecular weight marker.

Chromatographic profiles of cis-9, trans-11 conjugated linoleic acid (c9,t11-CLA) from Caco-2 cells. A) The 100-μM standard c9,t11-CLA; B) the level of c9,t11-CLA absorbed in the cells treated by c9,t11-CLA isomer; C) the level of c9,t11-CLA absorbed in the cells treated by β-LG binding CLA complex. AU=arbitrary units. Color version available in the online PDF.

The inhibition effect of β-LG binding cis-9, trans-11 conjugated linoleic acid (β-LG-CLA) complex on Caco-2 cells up to 72h. Color version available in the online PDF.