Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry

Distribution of oligosaccharides in colostrum samples collected during the first 60h of colostrum production from 6 individual Holstein-Friesian cows.

Distribution of acidic and neutral oligosaccharides (OS) in individual cows during the first 60h of colostrum production.

Extracted-ion chromatogram of ion m/z 636.23. Two sialyllactose (SL) isomers eluted at 8.0min (α2-6 SL) and 21.0min (α2-3 SL), respectively.

Area under the curve (AUC) distribution of 3′-sialyllactose (α2-3 SL) in individual cows during the first 60h of colostrum production.

Area under the curve (AUC) distribution of 6′-sialyllactose (α2-6 SL) in individual cows during the first 60h of colostrum production.

Distribution of 2 neutral N-acetylhexosamine (HexNAc)-containing oligosaccharides from individual cows during the first 60h of colostrum production. The sample from cow #2611 at 48h was excluded from the study because of the presence of contaminants.