Journal of Dairy Science
Volume 93, Issue 1 , Pages 19-26 , January 2010

Neutral glycosphingolipid content of ovine milk

  • L. Zancada

      Affiliations

    • Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, 37007 Salamanca, Spain
    • ,
  • F. Sánchez-Juanes

      Affiliations

    • Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, 37007 Salamanca, Spain
    • ,
  • J.M. Alonso

      Affiliations

    • Departamento de Bioquímica, Biología Molecular y Fisiología, Escuela Universitaria de Ingenierías Agrarias de Soria, Universidad de Valladolid, 42003 Soria, Spain
    • ,
  • P. Hueso

      Affiliations

    • Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, 37007 Salamanca, Spain
    • Corresponding Author InformationCorresponding author.

Received 10 June 2009 ,Accepted 30 September 2009.

  • Image Result

    Ovine milk neutral glycosphingolipids. A) High-performance thin-layer chromatography (HPTLC) separation of neutral glycosphingolipids: 1=neutral glycosphingolipid standard mixture; 2=ovine milk neutra

    Ovine milk neutral glycosphingolipids. A) High-performance thin-layer chromatography (HPTLC) separation of neutral glycosphingolipids: 1=neutral glycosphingolipid standard mixture; 2=ovine milk neutral glycosphingolipids. B) Densitometric analysis of an HPTLC plate.

  • Image Result
    Gas chromatogram profile of fatty acid methyl esters from ovine milk neutral glycosphingolipids.

    Gas chromatogram profile of fatty acid methyl esters from ovine milk neutral glycosphingolipids.

  • Image Result
    Adhesion of bacteria (Escherichia coli strains) to neutral glycosphingolipids separated by high-performance thin-layer chromatography. Standard chemical detection of neutral glycosphingolipids from ov

    Adhesion of bacteria (Escherichia coli strains) to neutral glycosphingolipids separated by high-performance thin-layer chromatography. Standard chemical detection of neutral glycosphingolipids from ovine milk with orcinol reagent. Immunostaining was carried out with an anti-E. coli polyclonal antibody. GlcCer=galactosylceramide; LacCer=lactosylceramide; Gb3=globotriaosylceramide; Gb4=globotetraosylceramide.

PII: S0022-0302(10)70260-5

doi: 10.3168/jds.2009-2476

Journal of Dairy Science
Volume 93, Issue 1 , Pages 19-26 , January 2010

Article Tools

* Image Usage & Resolution