Blocking of Pseudomonas aeruginosa and Chromobacterium violaceum lectins by diverse mammalian milks

Interactions of the reference plant lectin concanavalin A (Con A) with the diverse milks (using undialyzed and dialyzed samples), as analyzed by hemagglutination-inhibition and Western blot tests. The hemagglutination-inhibition values are presented as the percentages of those obtained in the PA-IIL with undialyzed human milk combination (100%). The Western blots were performed as described in the Materials and Methods section, using peroxidase-labeled Con A. The SEM of the second column, that of the dialyzed giraffe milk, is not noticeable in the figure (despite its inclusion in the data) because its dimension is very low (0.1) relative to the figure value scale.

Interactions of the diverse milks with Pseudomonas aeruginosa galactophilic lectin PA-IL, as demonstrated by blocking of the lectin activity in the hemagglutination-inhibition test and staining of the electrophoretically separated glycoproteins of the 6 most potent lectin-blocking milks in the Western blots by peroxidase-labeled PA-IL. The hemagglutination-inhibition values are presented as the percentages of those obtained in the PA-IIL with undialyzed human milk combination (100%). The Western blots were performed as described in the Materials and Methods section.

Interactions of the diverse milks with Pseudomonas aeruginosa fucophilic lectin PA-IIL, as demonstrated by blocking of the lectin activity in the hemagglutination-inhibition test and staining of the electrophoretically separated glycoproteins of the 6 most potent lectin-blocking milks in the Western blots by peroxidase-labeled PA-IIL. The hemagglutination-inhibition values are presented as the percentages of those obtained in the PA-IIL with undialyzed human milk combination (100%). The Western blots were performed as described in the Materials and Methods section.

Interactions of the diverse milks with Chromobacterium violaceum fucophilic lectin CV-IIL, as demonstrated by blocking of the lectin activity in the hemagglutination-inhibition test and staining of the electrophoretically separated glycoproteins of the 5 most potent lectin-blocking milks in the Western blots by peroxidase-labeled CV-IIL. The hemagglutination-inhibition values are presented as the percentages of those obtained in the PA-IIL with undialyzed human milk combination (100%). The Western blots were performed as described in the Materials and Methods section.