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Research-article| Volume 54, ISSUE 8, P1150-1161, August 1971

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In Vitro 15Nitrogen-tracer Technique for Some Kinetic Measures of Ruminal Ammonia1

  • Author Footnotes
    2 Present address: College of Agriculture, Department of Animal Production, University of Damascus, Damascus, Syria.
    M.F. Al-Rabbat
    Footnotes
    2 Present address: College of Agriculture, Department of Animal Production, University of Damascus, Damascus, Syria.
    Affiliations
    Department of Animal Science, University of California, Davis 95616
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  • R.L. Baldwin
    Affiliations
    Department of Animal Science, University of California, Davis 95616
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  • W.C. Weir
    Affiliations
    Department of Animal Science, University of California, Davis 95616
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  • Author Footnotes
    1 This paper is part of a dissertation submitted by M. F. Al-Rabbat to, the Graduate Division, University of California, Davis, in partial fulfillment of Requirements for the Ph.D. degree.
    2 Present address: College of Agriculture, Department of Animal Production, University of Damascus, Damascus, Syria.
Open ArchiveDOI:https://doi.org/10.3168/jds.S0022-0302(71)85993-3
In Vitro 15Nitrogen-tracer Technique for Some Kinetic Measures of Ruminal Ammonia1
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      Abstract

      To quantitate the several nitrogen fluxes which participate in the synthesis of ruminal microbes, an in vitro technique utilizing l5N ammonia was developed. The technique involves incubation of 15NH4-labelled rumen contents and analysis for 15N-ammonia and total ammonia N at specific time intervals during the incubation period.For a cow fed twice daily and a sheep fed at 2-hourly intervals, respectively, the technique yielded estimates of mean fractional turnover rates (Kam) of .226 and .314 per hour, and mean rates of ammonia nitrogen incorporation into cell materials (am) of 2.68 and 2.89 mg per hour per 50 of whole rumen digesta. The effect of rumen content donor on Kam was highly significant (P < 0.01); but the effect of am was not significant. For sheep fed alfalfa pellets continuously, about 9.2 g of ruminal microbiol cells per 100 g digestible organic matter fed are synthesized via ammonia nitrogen and about 61% of microbial nitrogen is derived from ammonia N. Presumably, 39% is derived from other nitrogen sources such as amino acid and peptide nitrogen.

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      Article info

      Publication history

      Received: December 3, 1970

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      DOI: https://doi.org/10.3168/jds.S0022-0302(71)85993-3

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      © 1971 American Dairy Science Association. Published by Elsevier Inc.

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