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Abstract
Microgard™, a commercially available fermented milk product containing antimicrobial metabolites, was a potent inhibitor for Gram-negative bacteria such as Pseudomonas, Salmonella, and Yersinia when 1% concentration was incorporated into agar media. Gram-positive Bacillus cereus, Staphylococcus aureus, and Listeria monocytogenes were insensitive to Microgard™. Kluyveromyces marxianus, an unidentified black yeast, and Penicillium expansum were partially suppressed, whereas Aspergillus niger and a yogurt spoilage yeast were tolerant to 5% Microgard™. Optimum activity of Microgard™ was at pH 5.3 and below; the concentration that gave complete inhibition depended upon the number of bacteria present as well as the genus tested. Blood agar base reversed the antagonistic activity of Microgard™ against Pseudomonas putida compared with plate count agar.
Key words
Abbreviation key:
BHI (brain-heart infusion), MIC (minimum inhibitory concentration), SPC (standard plate count)References
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Article info
Publication history
Accepted:
September 28,
1990
Received:
April 16,
1990
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Copyright
© 1991 American Dairy Science Association. Published by Elsevier Inc.
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