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Incorporation of Cholesterol into the Cellular Membrane of Lactobacillus acidophilus ATCC 431211

  • Author Footnotes
    2 Institute of Biotechnology, Korea University, Seoul, Korea.
    D.O. Noh
    Footnotes
    2 Institute of Biotechnology, Korea University, Seoul, Korea.
    Affiliations
    Department of Animal Science, Oklahoma State University, Stillwater 74078
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  • Author Footnotes
    3 Department of Animal Science, Korea University, Seoul, Korea.
    S.H. Kim
    Footnotes
    3 Department of Animal Science, Korea University, Seoul, Korea.
    Affiliations
    Department of Animal Science, Oklahoma State University, Stillwater 74078
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  • S.E. Gilliland
    Affiliations
    Department of Animal Science, Oklahoma State University, Stillwater 74078
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  • Author Footnotes
    1 Approved for publication by the Director, Oklahoma Agricultural Experiment Station. This research was supported under Project H-2013 and a grant from Centre International de Recherche, Daniel Carasso.
    2 Institute of Biotechnology, Korea University, Seoul, Korea.
    3 Department of Animal Science, Korea University, Seoul, Korea.
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      Abstract

      Cholesterol that was assimilated by Lactobacillus acidophilus ATCC 43121 was not metabolically degraded; most of it was recovered with the cells. Cells that were grown in the presence of cholesterol micelles and bile salts were more resistant to lysis by sonication than were those grown in their absence, suggesting a possible alteration of the cell wall or membrane. Cholesterol assimilation occurred during growth at pH 6.0 as well as during growth without pH control. Part of the cholesterol that was assimilated by cells was recovered in the membrane fractions of cells grown under both conditions. There was no difference in the amount taken up from cholesterol micelles that were prepared using dioleoyl L-α-phosphatidylcholine or distearoyl L-α-phosphatidylcholine. Thus, the type of fatty acid (unsaturated or saturated) in the phospholipid did not influence the assimilation. As the amount of Tween 80 in the growth media increased beyond 0.05%, cholesterol up-take decreased, and the amount of growth remained the same. The higher concentrations of Tween 80 may have adversely affected the permeability of the cells.

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