The Housekeeping Genes GAPDH and Cyclophilin are Regulated by Metabolic State in the Liver of Dairy Cows. R.P. Rhoads, C. McManaman, K.L. Ingvartsen, and Y.R. Boisclair J. Dairy Sci. 86:3423–3429.
It has come to our attention that Figures 1, 2 and 3 were poorly reproduced. Figures of appropriate quality are shown below. Readers should refer to JDS 86:3423 for legends.
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- The Housekeeping Genes GAPDH and Cyclophilin Are Regulated by Metabolic State in the Liver of Dairy CowsJournal of Dairy ScienceVol. 86Issue 11
- PreviewSteady-state levels of mRNA are often used to infer treatment effects on the levels of the corresponding protein. In addition, an internal standard RNA is usually measured to document specificity of treatment and to correct for intersample variation. Our objective was to evaluate whether glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and cyclophilin could be used as internal standards when studying changes in hepatic gene expression in dairy cows. Hepatic expression of GAPDH and cyclophilin was measured in 6 cows in late pregnancy (28 d prepartum) and early lactation (10 d postpartum).
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