Advertisement
Research-article| Volume 71, ISSUE 3, P620-626, March 1988

Download started.

Ok

Spontaneous Lipolysis in Bovine Milk: Combined Effects of Cream, Skim Milk, and Lipoprotein Lipase Activity

      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Total lipoprotein lipase activity has been studied in bovine milk. Lipase activity per milliliter milk decreased with decreasing yield in late lactation. Individual variation between cows was high, even at given milk yield. Evening samples were always higher in lipoprotein lipase activity than morning samples. This also applied to the degree of cold storage lipolysis (spontaneous lipolysis). However, the amount of liberated FFA per unit lipase was similar in morning and evening samples. Lipolysis in evening milk may be higher due to increased lipase. Methods to quantitate the influence of the skim milk fraction on lipolysis and the susceptibility of milk fat globules to lipolysis have been described previously. A linear multiple regression model that included these two parameters in addition to the total amount of lipoprotein lipase could explain 80 to 87% of cold storage lipolysis. Analysis including only one parameter resulted in low and nonsignificant coefficients. This demonstrated that cold storage lipolysis is a complex problem and that the properties of the fat globules and the skin milk, in addition to the total activity of lipoprotein lipase, are important.

      References

        • Ahrné L.
        • Björck L.
        Lipolysis and the distribution of lipase activity in bovine milk in relation to stage of lactation and time of milking.
        J. Dairy Res. 1985; 52: 55
        • Anderson M.
        Stability of lipoprotein lipase activity in bovine milk.
        J. Dairy Res. 1982; 49: 231
        • Castberg H.B.
        • Solberg P.
        The lipoprotein lipase and the lipolysis in bovine milk.
        Meieriposten. 1974; 63: 961
        • Chilliard Y.
        Variations physiologiques des activités lipasiques et de la lipolyse spontanée dans les laits de vache, de chévre et de femme: revue bibliographique.
        Le Lait. 1982; 62: 126
        • Claypool L.L.
        Studies on milk lipase activation.
        Diss. Abstr. Int. 1967 B. 1965; 27: 4438
        • Deeth H.C.
        • Fitz-Gerald C.H.
        Factors governing the susceptibility of milk to spontaneous lipolysis.
        Bull. Int. Dairy Fed. Doc. 1975; 86: 24
        • Deeth H.C.
        • Fitz-Gerald C.H.
        Lipolysis in dairy products: a review.
        Aust. J. Dairy Technol. 1976; 31: 53
        • Downey W.K.
        Risks from pre-and post-manufacture lipolysis.
        Bull. Int. Dairy Fed. Doc. 1980; 118: 4
        • Driessen F.M.
        • Stadhouders J.
        A study of spontaneous rancidity.
        Neth. Milk Dairy J. 1974; 28: 130
        • Egelrud T.
        • Olivecrona T.
        The purification of a lipoprotein lipase from bovine skim milk.
        J. Biol. Chem. 1972; 247: 6212
        • Jellema A.
        Note on susceptibility of bovine milk to lipolysis.
        Neth. Milk Dairy J. 1975; 29: 145
        • Landaas A.
        • Solberg P.
        A simple extraction-titration procedure for the determination of free fatty acids in milk.
        Meieriposten. 1974; 63: 497
        • Murphy J.J.
        • Conolly J.F.
        • Headon D.R.
        A study of factors associated with free fatty acid development in milk.
        Ir. J. Food Sci. Technol. 1979; 3: 131
        • Olivecrona T.
        Biochemical aspects of lipolysis in bovine milk.
        Bull. Int. Dairy Fed. Doc. 1980; 118: 19
        • Olivecrona T.
        • Bengtsson G.
        Borgström B. Brockman H.L. Lipases in milk. Elsevier, Amsterdam, Neth1984
        • Salih A.M.A.
        • Anderson M.
        Effect of diet and stage of lactation on bovine milk lipolysis.
        J. Dairy Res. 1979; 46: 623
      1. SAS Institute, Inc. 1982. SAS User's guide: statistics. Cary, NC.

        • Skipenes P.
        Variation in lipolysis in milk from individual cows.
        in: Proc. Eur. Assoc. Anim. Prod. 33rd Mtg., Leningrad1982
        • Suhren G.
        • Hamann J.
        • Tolle A.
        Free fatty acids in milk depending on the portion of the milking and the milking interval.
        in: 20th Int. Dairy Congr., Paris E1978: 79
        • Sundheim G.
        • Bengtsson-Olivecrona G.
        Lipolysis in milk induced by cooling or by heparin: comparisons of amount of lipoprotein lipase in the cream fraction and degree of lipolysis.
        J. Dairy Sci. 1985; 68: 589
        • Sundheim G.
        • Bengtsson-Olivecrona G.
        Iodine-125-labeled lipoprotein lipase as a tool to detect and study spontaneous lipolysis in bovine milk.
        J. Dairy Sci. 1986; 69: 1776
        • Sundheim G.
        • Bengtsson-Olivecrona G.
        Isolated milk fat globules as substrate for lipoprotein lipase. Studies of factors relevant to spontaneous lipolysis in milk.
        J. Dairy Sci. 1987; 70: 499
        • Sundheim G.
        • Bengtsson-Olivecrona G.
        Methods to assess the propensity of milk fat globules towards lipolysis and the ability of skim milk to inhibit lipolysis.
        J. Dairy Sci. 1987; 70: 2040
        • Sundheim G.
        • Bengtsson-Olivecrona G.
        Hydrolysis of bovine milk fat globules by lipoprotein lipase. Inhibition by proteins from the milk fat globule membrane.
        J. Dairy Sci. 1987; 70: 1815
        • Sundheim G.
        • Zimmer T.-L.
        • Astrup H.N.
        Induction of milk lipolysis by lipoprotein components of bovine blood serum.
        J. Dairy Sci. 1983; 66: 400
        • Tarassuk N.P.
        • Frankel E.N.
        The specificity of milk lipase. IV. Partition of the lipase system in milk.
        J. Dairy Sci. 1957; 40: 418

      Reference Added in Proof

        • Cartier P.
        • Chilliard Y.
        Effects of different skim milk fractions on activity of cow milk purified lipoprotein lipase.
        J. Dairy Sci. 1986; 69: 951