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Research-Article| Volume 72, ISSUE 3, P635-641, March 1989

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Quantification of Apolipoprotein A-1 in Cow Serum by Single Radial Immunodiffusion

  • Author Footnotes
    1 INRA, Theix.
    Andrzej Mazur
    Footnotes
    1 INRA, Theix.
    Affiliations
    Laboratoire des Maladies Métaboliques, INRA, Theix, 63 122 CEYRAT and UA CNRS 524, Laboratoire de Biochimie, Faculté de Médecine, Saint-Antoine 75 571 PARIS, Cedex 12, France
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  • Author Footnotes
    1 INRA, Theix.
    ,
    Author Footnotes
    2 Current address: INTA, EEA Rafaela, 2300 Rafaela, Argentina.
    Edgardo Marcos
    Footnotes
    1 INRA, Theix.
    2 Current address: INTA, EEA Rafaela, 2300 Rafaela, Argentina.
    Affiliations
    Laboratoire des Maladies Métaboliques, INRA, Theix, 63 122 CEYRAT and UA CNRS 524, Laboratoire de Biochimie, Faculté de Médecine, Saint-Antoine 75 571 PARIS, Cedex 12, France
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  • Author Footnotes
    1 INRA, Theix.
    Philippe Cardot
    Footnotes
    1 INRA, Theix.
    Affiliations
    Laboratoire des Maladies Métaboliques, INRA, Theix, 63 122 CEYRAT and UA CNRS 524, Laboratoire de Biochimie, Faculté de Médecine, Saint-Antoine 75 571 PARIS, Cedex 12, France
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  • Author Footnotes
    3 Faculté de Médecine Saint-Antoine, Paris.
    Maryse Ayrault-Jarrier
    Footnotes
    3 Faculté de Médecine Saint-Antoine, Paris.
    Affiliations
    Laboratoire des Maladies Métaboliques, INRA, Theix, 63 122 CEYRAT and UA CNRS 524, Laboratoire de Biochimie, Faculté de Médecine, Saint-Antoine 75 571 PARIS, Cedex 12, France
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  • Author Footnotes
    1 INRA, Theix.
    Yves Rayssiguier
    Footnotes
    1 INRA, Theix.
    Affiliations
    Laboratoire des Maladies Métaboliques, INRA, Theix, 63 122 CEYRAT and UA CNRS 524, Laboratoire de Biochimie, Faculté de Médecine, Saint-Antoine 75 571 PARIS, Cedex 12, France
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  • Author Footnotes
    1 INRA, Theix.
    2 Current address: INTA, EEA Rafaela, 2300 Rafaela, Argentina.
    3 Faculté de Médecine Saint-Antoine, Paris.
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      Abstract

      A single radial immunodiffusion assay was developed to determine the concentration of the major apolipoprotein A-I in bovine serum. Assay was performed using agarose gel plates with monospecific rabbit antiserum. Standards made with bovine high density lipoproteins or serum 10 times diluted were applied in 3-μl amounts to each well in the gel. The assay working range was 2 to 22 mg of apolipoprotein A-I. The intraassay and interassay CV were 3.7 and 4.9%, respectively. Using protein A-I. The intraassay and interassay CY were 3.7 and 4.9%, respectively. Using radial immunodiffusion assay, 23 serum samples from cows at various stages of lactation were analyzed. Apolipoprotein A-I was significantly increased in mid lactation compared with concentrations in early lactation or dry period.

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