Advertisement
Research-Article| Volume 72, ISSUE 10, P2464-2473, October 1989

Cloning and Sequencing of a Complementary Deoxyribonucleic Acid Coding for a Bovine αs1-Casein A from Mammary Tissue of a Homozygous B Variant Cow

  • Author Footnotes
    1 National Institutes of Health, Building 10, Room 9N-113, Bethesda, MD 20892.
    Robert A. McKnight
    Footnotes
    1 National Institutes of Health, Building 10, Room 9N-113, Bethesda, MD 20892.
    Affiliations
    Department of Food Science and Technology, University of California, Davis 95616
    Search for articles by this author
  • Rafael Jimenez-Flores
    Affiliations
    Department of Food Science and Technology, University of California, Davis 95616
    Search for articles by this author
  • Author Footnotes
    2 International Flavors and Fragrances, 1515 Highway 36, Union Beach, NJ 07735.
    Young Kang
    Footnotes
    2 International Flavors and Fragrances, 1515 Highway 36, Union Beach, NJ 07735.
    Affiliations
    Department of Food Science and Technology, University of California, Davis 95616
    Search for articles by this author
  • Author Footnotes
    3 New Zealand Dairy Research Institute, Palmerston North, NZ.
    Lawrence K. Creamer
    Footnotes
    3 New Zealand Dairy Research Institute, Palmerston North, NZ.
    Affiliations
    Department of Food Science and Technology, University of California, Davis 95616
    Search for articles by this author
  • Tom Richardson
    Affiliations
    Department of Food Science and Technology, University of California, Davis 95616
    Search for articles by this author
  • Author Footnotes
    1 National Institutes of Health, Building 10, Room 9N-113, Bethesda, MD 20892.
    2 International Flavors and Fragrances, 1515 Highway 36, Union Beach, NJ 07735.
    3 New Zealand Dairy Research Institute, Palmerston North, NZ.
Open ArchiveDOI:https://doi.org/10.3168/jds.S0022-0302(89)79386-3
Cloning and Sequencing of a Complementary Deoxyribonucleic Acid Coding for a Bovine αs1-Casein A from Mammary Tissue of a Homozygous B Variant Cow
Previous ArticleRennet Coagulation of Milk Retentates. 2. The Combined Effect of Heat Treatments and Protein Concentration
Next ArticleA Method for the Determination of α-Dicarbonyl Compounds1
      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      A cDNA clone for bovine αs1-casein variant A was isolated from a mammary gland cDNA library using a synthetic degenerate oligonucleotide probe. The largest Pst I insert containing an EcoR I site was sequenced. It contained 1090 base pairs, 47 in the 5′ noncoding region, 603 in the coding region and 440 in the 3′ noncoding region. The nucleotide sequence was compared with three published cDNA sequences for αs1-casein variant B. The most obvious difference was the absence of the 39 bases encoding the 13 amino acids that are present in die B variant but absent from the A variant. In addition, five other single base positions differed within individual codons among the four sequences at the third base for each codon, but this did not change the amino acids encoded. There were, however, a number of differences found in the 3′ noncoding region. The isolated cDNA was subjected to site-directed mutagenesis to replace a Val-Ile dipeptide with Phe-Phe to increase the chymosin sensitivity of the protein. When the milk proteins from mammary gland tissue extracts were typed, the αs1-casein A gene product was not detected.

      References

        • Bachvarova R.
        • De Leon V.
        • Johnson A.
        • Kaplan G.
        • Payntoo B.V.
        Changes in total RNA, polyadenylated RNA, and actin mRNA during meiotic maturationof mouse oocytes.
        Dev. Biol. 1985; 108: 325
        • Birnboim H.G.
        • Doly J.
        A rapid alkaline extraction procedure for screening lecombinant plasmid DNA.
        Nucleic Acids Res. 1979; 7: 1513
        • Chen E.T.
        • Seeburg P.H.
        Supercoil sequencing: a fast simple method for sequencing plasmid DNA.
        DNA. 1985; 4: 165
        • Eigel W.N.
        • Butler J.E.
        • Emstrom C.A.
        • Farrell Jr., H.M.
        • Harwalker N.R.
        • Jenness R.
        • Whitney Mc L.R.
        Nomenclature of proteins of cow's milk.
        J. Dairy Sci. 1984; 67: 1599
        • Gergen J.
        • Stem R.
        • Wensink P.
        Filter replicas and permanent collection of recombinant DNA plasmids.
        Nucleic Acids Res. 1979; 7: 2115

      6. Gillam, S., M. Zoller, and M Smith. 1985. Pages 159–161 in Recombinant DNA methodology. J.-A. Dillon, R. A. Nasim, and E. R. Nestmann. J. Wiley and Sons, New York, NY.

        • Gorodetskii S.I.
        • Zakhar’ev V.M.
        • Kyarshulite D.R.
        • Kapelinskaga T.V.
        • Skryabin K.G.
        Cloning and nucleotide sequence of cDNA for bovine αs1-casein.
        Biokhimya. 1986; 51: 1641
        • Grosclaude F.
        Le polymorphisme genetique des principles lactoproteines bovines.
        Inst Nail. Rech. Agron. Prod. Anim. 1988; 1: 5
        • Hobbs A.A.
        • Rosen J.M.
        Sequence of rat α-and γ-casein mRNA's: evolutionary comparison of the calcium dependent rat casein multigene family.
        Nucleic Acids Res. 1982; 10: 8079
        • Huarte J.
        • Belin D.
        • Vassalli A.
        • Strickland S.
        • Vassalli J.D.
        Meiotic maturation of mouse oocytcs triggers the translation and polyadenylation of dormantt issue-type plasminogen-activator mesaeoger-RNA.
        Genes Dev. 1987; 1: 1201
        • Huarte J.
        • Belin D.
        • Vassalli J.-D.
        Plasminogen activator in mouse and rat oocytes: induction during meiotic maturation.
        Cell. 1985; 43: 551
        • Jacobsen A.
        • Favreau M.
        Possible involvement of poly(A) in protein synthesis.
        Nucleic Acids Res. 1983; 11: 6353
        • Kang Y.C.
        • Richardson T.
        Molecular cloningand expression of bovine κ-casein in Escherichia coti.
        J. Dairy Sci. 1988; 71: 29
        • Manialis T.
        • Fritsch E.
        • Sanbrook J.
        Molecular cloning. A laboratory manual.
        Cold Spring Harbor Lab., New York, NY1982
        • Matyukov V.S.
        • Urnyshav A.P.
        Linkage of αs1- and κ-casein loci in cattle.
        Sov. Genet. 1980; 16: 572
        • Medrano J.F.
        • Sharrow L.
        Milk protein typing of bovine mammary gland tissue used to generate a cDNA library.
        J. Dairy Sci. 1989; 72 (in press)
        • Morinaga Y.
        • Franceschini T.
        • Inouye S.
        • Inouye M.
        Improvement of oligonucleotide-directed site specific mutagenesis using double stranded plasmid DNA.
        Biotechniques. 1984; 2: 636
        • Nagao M.
        • Maki M.
        • Sasaki R.
        • Chiba H.
        Isolation and sequence analysis of bovine αs1-casein cDNA clone.
        Agric. Biol. Chem. 1984; 48: 1663
        • Shapiro R.A.
        • Herrick D.
        • Manrow R.E.
        • Blinder D.
        • Jacobsoa A.
        Determinants of mRNA stability in Dictyostelium discoideum amoebae: differences in poly(A) tail length, ribosome loading, and mRNA size cannot account for the heterogeneity of mRNA decay rates.
        Mol. Cell. Biol. 1988; 8: 1957
        • Stewart A.F.
        • Willis I.M.
        • Mackinlay A.G.
        Nucleotide sequences of bovine αs1- and κ-casein cDNAs.
        Nucleic Acids Res. 1984; 12: 3895
        • Strickland S.
        • Huarte J.
        • Belin D.
        • Vassalli A.
        • Rickles R.J.
        • Vassalli J.-D.
        Antisense RNA directed against the 3’ noncoding region prevents dormantm RNA activation in mouse oocytes.
        Science. 1988; 241: 680
        • Thompson M.P.
        Phenotyping milk proteins: areview.
        J. Dairy Sci. 1970; 53: 1341
        • Thompson M.P.
        • Farrell H.M.
        Genetic variants of the milk proteins.
        in: Larson B.L. Smith V.R. Lactation. III. Academic Press, New York, NY1974: 118 (Ch. 2)
        • Yu-Lee L.-Y.
        • Richter-Mann L.
        • Couch C.H.
        • Stewart A.F.
        • Mackinlay A.G.
        • Rosen J.M.
        Evolution of the casein multigene family: conserved sequences in the 5’ flanking and exon regions.
        Nucleic Acids Res. 1986; 14: 1883

      Article info

      Publication history

      Accepted: March 6, 1989
      Received: October 3, 1988

      Identification

      DOI: https://doi.org/10.3168/jds.S0022-0302(89)79386-3

      Copyright

      © 1989 American Dairy Science Association. Published by Elsevier Inc.

      User license

      Elsevier user license |
      How you can reuse Information Icon
      Elsevier user license

      Permitted

      For non-commercial purposes:

      • Read, print & download
      • Text & data mine
      • Translate the article

      Not Permitted

      • Reuse portions or extracts from the article in other works
      • Redistribute or republish the final article
      • Sell or re-use for commercial purposes

      Elsevier's open access license policy

      ScienceDirect

      Access this article on ScienceDirect

      The content on this site is intended for healthcare professionals.


      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the Cookie Preference Center for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties.


      RELX