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Research-Article| Volume 72, ISSUE 10, P2683-2690, October 1989

Induction of Bovine Sperm Capacitation by TEST-Yolk Semen Extender1

  • A. Ijaz
    Affiliations
    Departments of Animal Science, and Large Animal Clinical Sciences, University of Minnesota, St. Paul 55108
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  • A.G. Hunter
    Affiliations
    Departments of Animal Science, and Large Animal Clinical Sciences, University of Minnesota, St. Paul 55108
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  • Author Footnotes
    1 Published as Paper Number 16,388 of the Scientific Journal Series of the Minnesota Agricultural Experiment Station on research conducted under Minnesota Agricultural Experiment Station Project Number 72 supported by Hatch funds.
Open ArchiveDOI:https://doi.org/10.3168/jds.S0022-0302(89)79410-8
Induction of Bovine Sperm Capacitation by TEST-Yolk Semen Extender1
Previous ArticleRelationship of Bull Fertility with Daughter Fertility and Production Traits in Holstein Dairy Cattle
Next ArticleEffect of Washing and Capacitating Media pH on Bull Sperm Motility, Acrosome Integrity, and Ability to Penetrate Zona-Free Hamster Oocytes1
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      Abstract

      Ejaculated bull semen was diluted 1:10 in the TEST-yolk buffer, cooled slowly to 4°C, and stored for up to 48 h. Aliquots were taken at 0, 4, 8, 16, 24, and 48 h and washed once or three times in bovine serum albumin-saline and the sperm pellets resuspended in this saline. Fertilization of zona-free hamster oocytes was used to assess sperm capacitation. Motility differed between samples washed once or three times (53.7 vs. 21.7%). Motility was highest at 4 h storage but did not differ between 16, 24, or 48 h of storage. More sperm without intact acrosomes were found at 4 h than at 0 h, but the percentage did not change further until after 24 h. Penetration of oocytes was not different between sperm washed once or three times (28.5 vs. 26.9%). No penetration occurred at 0 h, and highest penetration rates occurred at 4 and 8 h of storage (32.1 and 33.4%). Penetration rates at 16, 24, and 48 h were not different (25.3, 25.2, 22.5%). In conclusion, storage of bull sperm in TEST-yolk buffer for 4 to 48 h resulted in capacitation. Even though capacitation was induced by 4 h, at least 71% of the sperm population had not undergone an acrosome reaction by 48 h of storage. This may explain why penetrability was maintained over this period.

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      Article info

      Publication history

      Accepted: April 13, 1989
      Received: October 14, 1988

      Identification

      DOI: https://doi.org/10.3168/jds.S0022-0302(89)79410-8

      Copyright

      © 1989 American Dairy Science Association. Published by Elsevier Inc.

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