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Research-Article| Volume 72, ISSUE 10, P2691-2699, October 1989

Effect of Washing and Capacitating Media pH on Bull Sperm Motility, Acrosome Integrity, and Ability to Penetrate Zona-Free Hamster Oocytes1

  • A. Ijaz
    Affiliations
    Department of Animal Science and Large Animal Clinical Sciences, University of Minnesota, St. Paul 55108
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  • A.G. Hunter
    Affiliations
    Department of Animal Science and Large Animal Clinical Sciences, University of Minnesota, St. Paul 55108
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  • Author Footnotes
    1 Published as Paper Number 16,491 of the Scientific Journal Series of the Minnesota Agricultural Experiment Station on research conducted under Minnesota Agricultural Experiment Station Project Number 72 supported by Hatch funds.
Open ArchiveDOI:https://doi.org/10.3168/jds.S0022-0302(89)79411-X
Effect of Washing and Capacitating Media pH on Bull Sperm Motility, Acrosome Integrity, and Ability to Penetrate Zona-Free Hamster Oocytes1
Previous ArticleInduction of Bovine Sperm Capacitation by TEST-Yolk Semen Extender1
Next ArticleIdentification of the Capacitating Agent for Bovine Sperm in Egg Yolk-TEST Semen Extender1
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      Abstract

      Bovine-ejaculated sperm were washed thrice in bovine serum albumin-saline media, pH 7.2 to 8.4, and incubated at 37°C in Ca++-free Tyrode's media, pH 7.2 to 8.4, for 0, 2, 4, 6, and 8 h. Motility was highest when sperm were washed in pH 7.2 medium and incubated in pH 8.0 or 8.4 media. Motility remained above 50% until 8 h. Washing in pH 7.6, 8.0, or 8.4 media induced more acrosome reactions after incubation than washing at pH 7.2. Percentage of acrosome-reacted sperm increased at each successive time period. Sperm penetrated more oocytes at 4, 6, and 8 h when wash medium pH was fixed at 7.2 and capacitating media pH was raised at .4 unit increments from 7.2 to 8.4. When sperm were washed in pH 7.2 medium, the postincubation penetration rates peaked at 8 h. With wash media of pH 7.6, 8.0, or 8.4, the postincubation penetration rates peaked at 4 h and then gradually declined. In conclusion, the most effective system for capacitating bull sperm was a pH 7.6 wash followed by capacitation in pH 7.6 medium for 4 to 8 h and this system resulted in the highest penetration rates. Wash media pH hastened capacitation but was not a capacitating agent.

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      Article info

      Publication history

      Accepted: March 17, 1989
      Received: November 7, 1988

      Identification

      DOI: https://doi.org/10.3168/jds.S0022-0302(89)79411-X

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      © 1989 American Dairy Science Association. Published by Elsevier Inc.

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