Research-Article| Volume 72, ISSUE 10, P2718-2727, October 1989

Evaluation of On-Farm Milk Progesterone Tests to Determine Nonpregnant Cows and to Prevent Insemination Errors

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      The effect of using the on-farm milk progesterone test for early detection of nonpregnancy and for prevention of insemination errors was evaluated using modeling and simulation. The test was evaluated assuming low and high accuracies of progesterone measurement. For early detection of nonpregnancy, three testing schemes (one test on d 21 after service; two tests on d 21 and 23; three tests on d 19, 21, and 23) were compared against a control. Using the test extended the calving to first service interval by 3 d, resulted in 4 fewer d open, decreased the replacement rate from 20.4 to 18.4%, and increased the net return per cow per year by $13.00. Neither the accuracy of the test nor the testing scheme significantly affected the results. The use of the test to prevent insemination errors was evaluated for two error rates of estrus detection, 15 and 30%. Using the test resulted in .16 fewer services per conception, 3 additional d open, and $6.00 less in net return per cow per year. An interaction between using the test and the prevailing rate of errors in detection of estrus was found; the effect of the test was greater in herds with high prevalence of detection errors but was still unprofitable under the assumed costs and returns.


        • Bail P.J.H.
        • Jackson N.W.
        The fertility of dairy cows inseminated on the basis of milk progesterone measurements.
        Br. Vet. J. 1979; 135: 537
        • Bloomfield G.A.
        • Morant S.V.
        • Ducker M.J.
        A survey of reproductive performance in dairy herds Characteristics of the patterns of progesterone concentrations in milk.
        Anim. Prod. 1986; 42: 1
        • Esselmont R.J.
        • Bryant M.J.
        Oestrus behavior in a dairy herd.
        Vet. Rec. 1976; 99: 472
        • Fagan J.G.
        • Roche J.F.
        Reproductive activity in postpartum dairy cows based on progesterone concentrations in milk or rectal examination.
        Ir. Vet. J. 1986; 40: 124
        • Hoffmann B.
        • Gunzler O.
        • Hamburger R.
        • Schmidt W.
        Milk progesterone as a parameter for fertility control in cattle; methodological approaches and present status of application in Germany.
        Br. Vet. J. 1976; 132: 469
        • Hurnick J.F.
        • King G.J.
        • Robertson H.A.
        Estrus and related behavior in postpartum Holstein cows.
        Appl. Anim. Ethol. 1975; 2: 55
        • Kay G.W.
        • van Zyl J.P.
        • Naude R.T.
        Milk progesterone concentrations: an accurate early pregnancy diagnostic aid in dairy cattle.
        S. Afr. Tydskr. Veekd. 1985; 15. (4)
        • Nebel R.L.
        On-farm milk progesterone tests.
        J. Dairy Sci. 1988; 71: 1682
        • Nebel R.L.
        When should you use a cowside milk progesterone test? Hoard's Dairyman.
        June. 1986; : 10
        • Nebel R.L.
        Here's how cowside milk progesterone test works..
        Hoard's Dairyman. 1985; (May): 10
        • Oltenacu P.A.
        • Milligan R.A.
        • Rounsaville T.R.
        • Foote R.H.
        Modelling reproduction in a herd of dairy cattle.
        Agric. Syst. 1980; 5: 193
        • Oltenacu P.A.
        • Rounsaville T.R.
        • Milligan R.A.
        • Foote R.H.
        Systems analysis for designing reproductive management programs to increase production and profit in dairy herds.
        J. Dairy Sci. 1981; 64: 2096
        • Oltenacu P.A.
        • Smith R.D.
        • Lednor A.
        Control of the reproductive process for optimum performance in dairy herds. A case study for systems analysis.
        in: Proc. Thirty-Fifth Anim. Natl. Breeders Roundtable, St. Louis, MO1986
        • Pennington J.A.
        • Albright J.L.
        • Callahan C.J.
        Relationships of sexual activities in estrous cows to different frequencies of observation and pedometer measurements.
        J. Dairy Sci. 1986; 69: 2925
        • Reimers T.J.
        • Smith R.D.
        • Newman S.K.
        Management factors affecting reproductive performance of dairy cows in the North Eastern United States.
        J. Dairy Sci. 1985; 68: 963
        • Rounsaville T.R.
        • Oltenacu P.A.
        • Milligan M.A.
        • Foote R.H.
        Effects of heat detection, conception rate and culling policy on reproductive performance in dairy herds.
        J. Dairy Sci. 1979; 62: 1435
        • Van de Wiel D.F.M.
        • Van Eldik J.
        • Koops W.
        • Postma A.
        • Oldenbroek J.K.
        Fertility control in cattle by use of the milk progesterone test.
        Tijdschr. Diergeneeskd. 1978; 103: 91
        • Wimpy T.H.
        • Chang C.F.
        • Estergreen V.L.
        • Hillers J.K.
        Milk progesterone enzyme immunoassay: modifications and a field trial for pregnancy detection in dairy cows.
        J. Dairy Sci. 1986; 69: 1115