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Research-Article| Volume 72, ISSUE 10, P2728-2732, October 1989

Evaluation of the Rapid Strep System for Identification of Gram-Positive, Catalase-Negative Cocci Isolated from Bovine Intramammary Infections1

  • Jeffrey L. Watts
    Affiliations
    Mastitis Research Laboratory, Hill Farm Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Route 1, Box 10, Homer 71040
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  • Author Footnotes
    1 Approved for publication by the Director of the Louisiana Agricultural Experiment Station as Manuscript Number 88-80-2525.
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      Abstract

      The Rapid Strep system (Analytab Products, Plainview, NY) was used to identify 199 gram-positive, catalase-negative cocci isolated from bovine intramammary infections. The system accurately identified 88.4% of isolates. The system identified 100% of 46 Streptococcus agalactiae, 100% of 48 Streptococcus dysgalactiae, 54.5% of 11 Streptococcus equinus, and 96.2% of 53 Streptococcus uberis isolates. Enterococcus spp. were identified correctly 83.3% of the time. One of 4 Streptococcus saccharolyticus strains was identified as Streptococcus bovis, the previous classification for this organism, and 8 Streptococcus equi ssp. equi strains were misidentified as Streptococcus dysgalactiae. The Rapid Strep system was determined to be an acceptable alternative to conventional methods for identification of gram-positive, catalase-negative cocci isolated from bovine intramammary infections.

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