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Article| Volume 73, ISSUE 5, P1225-1231, May 1990

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Minimum Inhibitory Concentrations and Disk Diffusion Zone Diameter for Selected Antibiotics Against Streptococci Isolated from Bovine Intramammary Infections1

  • W.E. Owens
    Affiliations
    Hill Farm Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Route 1, Box 10, Homer 71040
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  • J.L. Watts
    Affiliations
    Hill Farm Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Route 1, Box 10, Homer 71040
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  • B.B. Greene
    Affiliations
    Hill Farm Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Route 1, Box 10, Homer 71040
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  • C.H. Ray
    Affiliations
    Hill Farm Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Route 1, Box 10, Homer 71040
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  • Author Footnotes
    1 Approved for publication by the Director of he Louisiana Agricultural Experiment Station as Manuscript Number 89-8@3352.
Open ArchiveDOI:https://doi.org/10.3168/jds.S0022-0302(90)78786-3
Minimum Inhibitory Concentrations and Disk Diffusion Zone Diameter for Selected Antibiotics Against Streptococci Isolated from Bovine Intramammary Infections1
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      Abstract

      The antimicrobial susceptibility to 11 antibiotics was determined of 358 streptococcal isolates representing six species was determined. All isolates were from milk samples obtained from clinical or subclinical cases of bovine mastitis. Agar disk diffusion zone diameters and minimum inhibitory concentrations were obtained for all isolates. Of the antibiotics tested that are available for mastitis therapy, cephalothin, penicillin, ampicillin, novobiocin, and erythromycin demonstrated acceptable activity against nonenterococcal streptococci, but only penicillin and ampicillin demonstrated moderate activity against Enterococcus faecalis. Enterococcus faecalis demonstrated substantial resistance to cephalothin, erythromycin, gentamicin, streptomycin, and tetracycline, while the nonenterococcal streptococci demonstrated substantial resistance to gentamicin, streptomycin, and tetracycline. Streptococcus uberis demonstrated greater antibiotic resistance than did Streptococcus dysgalactiae, while Aerococcus viridans demonstrated the greatest overall resistance of the nonenterococcal species.

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      References

        • Brown R.W.
        • Barnum D.A.
        • Jasper D.E.
        • McDonald J.S.
        • Schultze W.D.
        In Microbiological procedures for use in the diagnosis of bovine mastitis. 2nd ed. Natl. Mastitis Counc, Inc., Arlington, VA1981
        • Davidson J.N.
        Antibiotic resistance patterns of bovine mastitis pathogens.
        in: Proc. 19th Annu. Mtg. Natl. Mastitis, Counc., Inc., Arlington, VA1980 (181)
        • Dodd F.H.
        Mastitis–progress on control.
        J. Dairy Sci. 1983; 66: 1773
        • Dodd F.H.
        • Westgarth D.R.
        • Griffin T.F.
        Strategy of mastitis control.
        J. Am. Vet. Med. Assoc. 1977; 170: 1124
        • Hinckley L.S.
        • Benson R.H.
        • Post J.E.
        • DeCloux J.C.
        Antibiotic susceptibility profiles for mastitis treatment.
        J. Am. Vet. Med. Assoc. 1985; 187: 709
        • Jasper D.D.E.
        Antimicrobial susceptibility of staphylococci isolated from bovine mastitis.
        California Vet. (Oct.). 1972; l
        • McDonald J.S.
        • Anderson A.J.
        Antibiotic sensitivity of Staphylococcus aweus and coagulase-negative staphylococci isolated from infected bovine mammary glands.
        Cornell Vet. 1981; 71: 391
        • McDonald J.S.
        • McDonald T.J.
        • Anderson A.J.
        Antimicrobial sensitivity of aerobic gram-negative rods isolated from bovine udder infections.
        Am. J. Vet. Res. 1977; 38: 1503
        • McDonald J.S.
        • McDonald T.J.
        • Stark D.R.
        Antibiograms of streptococci isolated from bovine intramammary infections.
        Am. J. Vet. Res. 1976; 37: 1185
        • Oliver S.P.
        • Mitchell B.A.
        Prevalence of mastitis pathogens in herds participating in a mastitis control program.
        J. Dairy Sci. 1984; 67: 2436
        • Owens W.E.
        • Nickerson S.C.
        Antibiotic levels in milk and mammary tissues during various treatment regimens for bovine mastitis.
        Agri-Practice. 1989; 10: 10
        • Owens W.E.
        • Watts J.L.
        Antimicrobial susceptibility and β-lactamase testing of staphylococci isolated from dairy herds.
        J. Dairy Sci. 1988; 71: 1934
        • Owens W.E.
        • Watts J.L.
        • Boddie R.L.
        • Nickerson S.C.
        Antibiotic treatment of mastitis: comparison of intramammary and intramammary plus intra muscular therapies.
        J. Dairy Sci. 1988; 71: 3143
        • Paape M.J.
        • Wergin W.P.
        • Guidry A.J.
        • Pearson R.E.
        Leukocytes–second line of defense against invading mastitis pathogens.
        J. Dairy Sci. 1979; 62: 135
      15. Performance standards for antimicrobic disc susceptibility tests. 4th ed. Tentative standard. Natl. Comm. Clin. Lab. Standards, Villanova, PA.1984
        • Soback S.
        Therapeutic success or failure in mastitis therapy - a pharmacokinetic approach.
        in: Proc. 26th Annu. Mtg. Natl. Mastitis Counc, Inc., Arlington, VA1987: 1
        • Sordillo L.M.
        • Nickerson S.C.
        • Akers R.M.
        • Oliver S.P.
        Secretion composition during bovine mammary involution and the relationship with mastitis.
        Int. J. Biochem. 1987; 19: 1165
        • Washington J.A.
        Susceptibility tests; agar dilution.
        in: Lennette E.H. Ballows A. Hausler W.J. Shadomy H.J. Manual of clinical microbiology.4th ed. Am. Soc. Microbiol., Washington DC1985: 967
        • Watts J.L.
        Etiological agents of bovine mastitis.
        Vet. Microbiol. 1988; 16: 41
        • Watts J.L.
        Characterization and identification of streptococci isolated from bovine mammary glands.
        J. Dairy Sci. 1988; 71: 1616
        • Watson D.L.
        Immunological functions of the mammary gland and its secretion. Comparative review.
        Aust. J. Biol. Sci. 1980; 33: 403

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      Publication history

      Accepted: December 11, 1989
      Received: July 17, 1989

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      DOI: https://doi.org/10.3168/jds.S0022-0302(90)78786-3

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      © 1990 American Dairy Science Association. Published by Elsevier Inc.

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