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Article| Volume 74, ISSUE 4, P1145-1150, April 1991

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Inhibition of Clostridium tyrobutyricum by Bacteriocin-Like Substances Produced by Lactic Acid Bacteria

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      Abstract

      Lactic acid bacteria were selected for their inhibitory activity against Clostridium tyrobutyricum under conditions that eliminate the effects of lactic acid and hydrogen peroxide. Four strains were isolated belonging to the species Lactococcus lactis ssp. lactis. The sensitivity of the inhibitory substances to pronase and trypsine indicates that they are proteins or peptides different from nisin. Their resistance to phospholipase D indicates that they are also different from lactostrepcin. The inhibitory substances are produced during the exponential phase of growth. Their activity is bactericidal and directed toward some strains of Clostridium tyrobutyricum, Lactobacillus helveticus, and Streptococcus thermophilus, but strains used as dairy starters, Lactobacillus lactis, Streptococcus thermophilus, and Propionibacterium shermanii, are not all affected by the inhibition.

      Key words

      Abbreviation Key:

      MMRS (modified MRS medium), RCM (reinforced clostridial agar medium)

      References

        • Anderson R.
        Inhibition of Staphylococcus aureus and spheroplasts of Gram-negative bacteria by anantagonistic compound produced by a strain of Lac-tobacillus plantarum.
        Int. J. Food Microbiol. 1986; 3: 149
        • Barefoot S.R.
        • Klaenhammer T.R.
        Detection and activity of Lactacin B, a bacteriocin produced by Lactobacillus acidophilus.
        Appl. Environ. Microbiol. 1983; 45: 1808
        • Bergere J.L.
        • Sys T.
        • Vassal L.
        Bacteries lactiqucs susceptibles d’inhiber la croissance de Clostridium tyrobutyricum en culture et dans le fromage.
        Lait. 1978; 575: 215
        • Bergere J.L.
        • Hennier J.
        Étude des facteurscontrölant la croissance et la sporulation de Clostridium tyrobutyricum.
        Ann. Institut Pasteur. 1965; 109: 80
        • Bourgeois C.M.
        • Le Pare O.
        • Abgrall B.
        • Cleret J.J.
        Membrane filtration of milk for countingspores of Clostridium tyrobutyricum.
        J. Dairy Sci. 1984; 67: 2493
        • DaescheL M.A.
        • Klaenhammer T.R.
        Association of 13.6 megadalton plasmid in Pediococcus pentosaceus with bacteriocin activity.
        Appl. Environ. Microbiol. 1985; 50: 1538
        • Davey G.P.
        • Richardson B.C.
        Purification and some properties of Diplococcin from Streptococcus cremoris 346.
        Appl. Environ. Microbiol. 1981; 41: 84
        • Deklerk H.C.
        • Smit J.A.
        Properties of a Lactobacillus fermenti bacteriocin.
        J. Gen. Microbiol. 1967; 48: 309
        • Geis A.
        • Singh J.
        • Teuber M.
        Potential of lactic streptococci to produce bacteriocin.
        Appl. Environ. Microbiol. 1983; 45: 205
        • Gonzales C.F.
        • Kunka B.S.
        Plasmid-associated bacteriocin production and sucrose fermentation in Pediococcus acidilactici.
        Appl. Environ. Microbiol. 1987; 53: 2534
        • Graham D.C.
        • McKay L.L.
        Plasmid DNA in strains of Pediococcus cerevisiae and Pediococcuspentosaceus.
        Appl. Environ. Microbiol. 1985; 50: 532
        • Gross E.
        • Morrel J.L.
        The structure of nisin.
        J. Am. Chem. Soc. 1970; 93: 4634
        • Hirsch A.
        • McClintoch M.
        • Mocquot G.
        Observation on the influence of inhibitory substances produced by the lactobacilli of Gruyere cheese on the development of anaerobic sporeformers.
        J. Dairy Res. 1952; 19: 179
        • Hurst A.
        Nisin and other inhibitory substances from lactic acid bacteria.
        in: Branen A.L. Davidson P.M. Antimicrobials in food. Marcel Dekker, Inc., New York, NY1983: 327
        • Joerger M.C.
        • Klaenhammer T.R.
        Characterization and purification of Helveticin J and evidence for a chromosomally determined bacteriocin producedby Lactobacillus helveticus 481.
        J. Bacteriol. 1986; 167: 439
        • Kordel M.
        • Sahl H.G.
        Susceptibility of bacterial eukaryotic and artificial membranes to the disruptive action of (he canonic peptides Pep 5 and nisin.
        Fed. Eur. Microbiol. Soc. Microbiol. Lett. 1986; 34: 136
        • Kozak W.
        • Bardowski J.
        • Dobrzanski W.T.
        Lactostrepcins-acid bacteriocins produced by lactics treptococci.
        J. Dairy Res. 1978; 45: 247
        • Pucci M.S.
        • Vedamuthu E.R.
        • Kunka B.S.
        • Vandenbergh P.A.
        Inhibition of listeria monocyto-genes by using Bacteriocin PA 1. produced by Pediococcus acidilactici PAC 1.0.
        Appl. Environ. Microbiol. 1988; 54: 2319
        • Ruth E.
        • Sahl H.G.
        Mode of actionof peptide antibiotic nisin and influence on the membrane potential of whole cells and on cytoplasmic andartificial membrane vesicles.
        Antimicrob. Agents Chemother. 1985; 27: 841
        • Tagg J.R.
        • Dajani A.S.
        • Wannamaker L.W.
        Bacteriocins of gram positive bacteria.
        Bacteriol. Rev. 1976; 40: 722
        • Upreti G.C.
        • Hinsdill R.D.
        Isolation and characterization of bacteriocin from a homofennentative Lactobacillus.
        Antimicrob. Agents Chemother. 1973; 4: 487
        • Whitehead H.R.
        A substance inhibiting bacterial growth produced by certain strains of lactic streptococci.
        Biochem. J. 1933; 27: 1793