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Article| Volume 74, ISSUE 4, P1218-1226, April 1991

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Bovine Lactoferrin Receptors in Staphylococcus aureus Isolated from Bovine Mastitis

Open ArchiveDOI:https://doi.org/10.3168/jds.S0022-0302(91)78277-5
Bovine Lactoferrin Receptors in Staphylococcus aureus Isolated from Bovine Mastitis
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      Abstract

      A total of 103 Staphylococcus aureus strains isolated from bovine mastitis were tested for bovine lactoferrin binding in a 125I-labeled protein binding assay. More than 85% of the strains demonstrated high to moderate and a few showed little or no binding. Bovine lactoferrin binding to S. aureus cells was high when grown on blood, nutrient, or proteose-peptone agar, but the binding capacity was low with cells grown on salt rich media, in skim milk, or in broth. The kinetics of 125I-labeled bovine lactoferrin binding required approximately 90 min for complete saturation with optimal interaction in the pH range 4.0 to 7.0. The lactoferrin-staphylococci interaction was specific with a high affinity (association constant, Ka 14 × 106 L/mol). Scatchard plot analysis estimated the number of binding sites per cell at 7200 on strain SA-340. Unlabeled bovine lactoferrin effectively displaced the binding of the labeled ligand to strain SA-340 in a dose-dependent manner. Bovine lactoferrin binding was inhibited or displaced by human lactoferrin. Various plasma, connective tissue, or mucosal secretory proteins tested did not inhibit lactoferrin-staphylococci interaction. Bovine lactoferrin binding components on SA-340 were resistant to glycolytic enzymes and moderately susceptible to proteolytic digestion. Two proteins with an estimated molecular weight of approximately 92 and 67 kDa were identified as bovine lactofenin binding components of S. aureus strain SA-340.

      Key words

      Abbreviation key:

      bLf (bovine lactoferrin), hLf (human lactoferrin), HRPO (horseradish peroxidase), Lf (lactoferrin), PBS (phosphate-buffered saline), PMN (polymorphonuclear leukocytes)

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      Article info

      Publication history

      Accepted: November 1, 1990
      Received: June 18, 1990

      Identification

      DOI: https://doi.org/10.3168/jds.S0022-0302(91)78277-5

      Copyright

      © 1991 American Dairy Science Association. Published by Elsevier Inc.

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