This paper is only available as a PDF. To read, Please Download here.
Abstract
Sixteen ejaculates were collected, four each from four bulls, using artificial vaginas with polyethylene or rubber liner collection cones in a crossover design experiment. The ejaculates were diluted with egg yolk-citrate extender at pH 6.4 or 7.2, cooled, glycerolated, equilibrated, packaged in .5-ml French straws, frozen in nitrogen vapor, and stored in liquid nitrogen. Thirty frozen straws from each ejaculate were thawed rapidly (46.5°C for 12 s), pooled, and then incubated at 46.5°C for periodic evaluation of progressive motility, differential staining, and acrosome morphology under thermal stress conditions. The postthaw motility of spermatozoa and percentage of unstained cells were higher both when collected in polyethylene than in rubber and when extended at pH 7.2 vs. 6.4, but no interaction was found between liner collection cone composition and pH for postthaw motility. Retention of spermatozoan motility during incubation under thermal stress was greater for cells collected in polyethylene, but not different due to pH. Neither pH nor composition of liner collection cone had an effect on postthaw acrosomal scores, but the time required for a 50% increase in severely damaged acrosomes was greater for spermatozoa collected in polyethylene than in rubber liner collection cones.
Key words
References
- Rapid methods for estimating the quality of bull semen.J. Dairy Sci. 1943; 26: 483
- Biological testing of the toxicity of rubber used in artificial vaginas with boar semen.Nord. Vet Med. 1962; 14: 689
- The effect of exposing media to syringes on the viability of bovine embryos.Theriogenology. 1986; 25 (Abstr.): 142
- Robber toxicity for equine spermatozoa.J. Anim. Sci. 1989; 67 (Abstr.): 12
- Toxicity of artificial vagina liners for bovine spermatozoa.Theriogenology. 1988; 29: 1207
- Influence of pH on survival and fertility of bull sperm.J. Dairy Sci. 1964; 47: 807
- The artificial insemination and embryo transfer of dairy and beef cattle.Interstate Printers and Publishers, Inc, Danville, IL1987
- Some metabolic measurements of diluted bovine semen adjusted to various pH.J. Dairy Sci. 1953; 36 (Abstr.): 578
- The effect of artificial vagina liners on livability of stallion spermatozoa.Equine Vet. Sci. 1987; 7: 226
- In vitro screening studies of the lexicological testing of synthetic biomaterials.Polym. Med. 1980; 19: 193
- Effects of liner composition, cooling profile and time of glycerolization on survival of bovine spermatozoa.Theriogenology. 1989; 31: 623
SAS® User's Guide: Statistics, Version 5 Edition. 1985. SAS Inst, Inc., Cary, NC.
- Effect of polymers at 5°C on postthaw motility of bovine spermatozoa.J. Dairy Sci. 1988; 71 (Abstr.): 221
- Effect of plastic disposable syringes on development of mouse embryos in culture.Theriogenology. 1986; 25 (Abstr.): 205
- Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa.Vet Rec. 1975; 97: 12
Article info
Publication history
Accepted:
November 26,
1990
Received:
June 13,
1990
Identification
Copyright
© 1991 American Dairy Science Association. Published by Elsevier Inc.
User license
Elsevier user license | How you can reuse 
Elsevier's open access license policy
Elsevier user license
Permitted
For non-commercial purposes:
- Read, print & download
- Text & data mine
- Translate the article
Not Permitted
- Reuse portions or extracts from the article in other works
- Redistribute or republish the final article
- Sell or re-use for commercial purposes
Elsevier's open access license policy
