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Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition

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      Abstract

      There is a need to standardize the NDF procedure. Procedures have varied because of the use of different amylases in attempts to remove starch interference. The original Bacillus subtilis enzyme Type IIIA (XIA) no longer is available and has been replaced by a less effective enzyme. For fiber work, a new enzyme
      The heat stable amylase, formerly Number 5426, has been changed by Sigma as of July 1991. The original procedure required .2 ml of this enzyme. The replacement. Number A3306, is four times stronger, and 50 μl are used per sample.
      1The heat stable amylase, formerly Number 5426, has been changed by Sigma as of July 1991. The original procedure required .2 ml of this enzyme. The replacement. Number A3306, is four times stronger, and 50 μl are used per sample.
      has received AOAC approval and is rapidly displacing other amylases in analytical work. This enzyme is available from Sigma (Number A3306; Sigma Chemical Co., St. Louis, MO). The original publications for NDF and ADF (43, 53) and the Agricultural Handbook 379 (14) are obsolete and of historical interest only. Up to date procedures should be followed. Triethylene glycol has replaced 2-ethoxyethanol because of reported toxicity. Considerable development in regard to fiber methods has occurred over the past 5 yr because of a redefinition of dietary fiber for man and monogastric animals that includes lignin and all polysaccharides resistant to mammalian digestive enzymes. In addition to NDF, new improved methods for total dietary fiber and nonstarch polysaccharides including pectin and β-glucans now are available. The latter are also of interest in rumen fermentation. Unlike starch, their fermentations are like that of cellulose but faster and yield no lactic acid. Physical and biological properties of carbohydrate fractions are more important than their intrinsic composition.

      Key words

      Abbreviation key:

      AD (acid detergent), AIA (acid-insoluble ash), ND (neutral detergent), NSC (nonstructural carbohydrates), NSP (nonstarch polysaccharides)

      References

        • Akin D.E.
        Evaluation by electron microscopy and anaerobic culture of types of rumen bacteria associated with digestion of forage cell walls.
        Appl. Environ. Microbiol. 1980; 39: 242
        • Allen M.S.
        • McBurney M.I.
        • Van Soest P.J.
        Cation-exchange capacity of plant cell walls at neutral pH.
        J. Sci. Food Agric. 1985; 36: 1065
        • Aman P.
        • Hesselman K.
        An enzyme method for analysis of total mixed linkage beta glucans in cereal grains.
        J. Cereal Sci. 1985; 3: 231
      1. Aspinall, G. O. 1983. Structural chemistry of some nonstarchy polysaccharides of carrots and apples. Page 33 in Unconventional sources of fiber. Furda, ed. Am. Chem. Soc., Washington, DC. Symp. Sec. 214.I.

        • Bae D.H.
        • Welch J.G.
        • Smith A.M.
        Forage intake and rumination by sheep.
        J. Anim. Sci. 1979; 49: 1292
        • Blumencrantz N.
        • Asboe-Hansen G.
        A new method for quantitative determination of uronic acids.
        Anal. Biochem. 1973; 54: 484
      2. Bucher, A. C. 1984. A comparison of solvent systems for extraction of pectic substances from fruits and vegetables. M.S. Thesis, Cornell Univ., Ithaca, NY.

        • Conrad H.R.
        • Weiss W.P.
        • Odwongo W.O.
        • Shockey W.L.
        Estimating net energy lactation from components of cell solubles and cell walls.
        J. Dairy Sci. 1984; 67: 427
        • Englyst H.N.
        • McFarlane G.T.
        Breakdown of resistant and readily digestible starch by human gut bacteria.
        J. Sci. Food Agric. 1986; 37: 699
        • Fonnesbeck P.V.
        Estimating nutritive value from chemical analyses.
        in: Fonnesbeck P.V. Harris L.E. Kearl L.C. 1st Int. Symp. Feed Composition, Animal nutrient requirements and computerization of diets. Utah Slate Univ., Logan1976: 219
        • Fox D.G.
        • Sniffen C.J.
        • O’Connor J.D.
        • Russell J.B.
        • Van Soest P.J.
        The Cornell net carbohydrate and protein system or evaluating cattle diets. Search Agric.
        Cornell Univ. Agric., Ithaca, NY1990 (Exp. Stn. No. 34)
        • French D.
        Chemical and physical properties of starch.
        J. Anim. Sci. 1973; 37: 1048
        • Giger S.
        • Dorleans M.
        • Sauvant D.
        Adaptation of the Van Soest method to a routine determination of concentrate feedstuffs. Comm. Eur. Commun. Workshop Methodol. Anal. Feedingstuffs Ruminants. European Van Soest Ring Test. Rep. meeting to discuss analytical results.
        Ministry of Agriculture, Fisheries, and Food, Slough Laboratory, Lond., Engl.1981
        • Goering H.K.
        • Van Soest P.J.
        Forage fiber analyses (apparatus, reagents, procedures, and some applications).
        Agric. ARS-USDA, Washington, DC1970 (Handbook No. 379)
        • Hale W.H.
        Influence of processing on the utilization of grains (starch) by ruminants.
        J. Anim. Sci. 1973; 37: 1075
        • Jeraci J.L.
        • Van Soest P.J.
        Improved methods for analysis and biological characterization of fiber.
        Adv. Exp. Med. Biol. 1990; 270: 245
        • Jeraci J.L.
        • Hernandez T.H.
        • Robertson J.B.
        • Van Soest P.J.
        New and improved procedure for neutral-detergent fiber.
        J. Anim. Sci. 1988; 66 (Abstr.): 351
        • Jeraci J.L.
        • Lewis B.A.
        Determination of soluble fiber components: (l->3; l->4)-β-D-glucans and pectins.
        Anim. Feed Sci. Technol. 1989; 23: 15
        • Jeraci J.L.
        • Lewis B.A.
        • Van Soest P.J.
        • Robertson J.B.
        New urea enzymatic dialysis procedure for total dietary fiber.
        J. Assoc. Offic. Anal. Chem. 1989; 72: 677
        • Keijbets M.J.H.
        • Pilnik W.
        Some problems in the analysis of pectin in potato tuber issue.
        Potato Res. 1974; 17: 169
        • King R.H.
        • Taverner M.R.
        Effect of time of digestion with NDF solution on the yield of fibre from various feedstuffs.
        Anim. Prod. 1975; 21: 284
        • MacGregor C.A.
        • Stokes M.R.
        • Hoover W.H.
        • Leonard H.A.
        • Junkins Jr., L.L.
        • Sniffen C.J.
        • Mailman R.W.
        Effect of dietary concentration of total nonstructural carbohydrates on energy and nitrogen metabolism and milk production of dairy cows.
        J. Dairy Sci. 1983; 66: 39
        • Marquis R.E.
        • Mayzel K.
        • Carstensen E.L.
        Cation exchange in cell walls of gram-positive bacteria.
        Can. J. Microbiol. 1976; 22: 975
        • Marshall K.C.
        Bacterial adhesion in natural environments.
        in: Berkely R.C.W. Lynch J.M. Melling J. Rutter P.R. Microbial adhesion to surfaces. Soc. Chem. Ind, London, Engl.1980: 187
        • Mascarenhas-Ferreira A.
        • Kerstens J.
        • Gasp C.H.
        The study of several modifications of the neutral-detergent fibre procedure.
        Anim. Feed Sci. Technol. 1983; 9: 19
        • McBuraey M.I.
        • Allen M.S.
        • Van Soest P.J.
        Praseodymium and copper cation-exchange capacities of neutral-detergent fibres relative to composition and fermentation kinetics.
        J. Sci. Food Agric. 1986; 37: 666
        • McBurney M.I.
        • Van Soest P.J.
        • Chase L.E.
        Cation exchange capacity of various feedstuffs in ruminant rations.
        in: Proc. Cornell Nutr. Conf. Feed Manuf, Syracuse, NY1981: 16
        • McBurney M.I.
        • Van Soest P.J.
        • Chase L.E.
        Cation-exchange capacity and buffering capacity of neutral-detergent fibres.
        J. Sci. Food Agric. 1983; 34: 910
        • McQueen R.E.
        • Nicholson J.W.G.
        Modification of the neutral-detergent fiber procedure for cereals and vegetables by using an alpha-amylase.
        J. Assoc. Offic. Anal. Chem. 1979; 62: 676
      3. Mertens, D. R. 1973. Application of theoretical mathematical models to cell wall digestion and forage intake in ruminants. Ph.D. Diss., Cornell Univ., Ithaca, NY.

        • Mertens D.R.
        Balancing carbohydrates in dairy rations.
        in: Proc. 2nd Large Herd Conf. Cornell Univ., Dep. Anim. Sci., Ithaca, NY1988: 150
        • Mongeau R.
        • Brassard R.
        Determination of neutral-detergent fiber in breakfast cereals: pentose, hemicellulose, cellulose and lignin content.
        J. Food Sci. 1982; 47: 550
        • National Research Council
        Nutrient requirements of dairy cattle.
        6th rev. ed. Natl. Acad. Press, Washington, DC1988
      4. Nocek, J., D. G. Braund, R. L. Steele, and C. H. McGregor, inventors. 1986. Agway, assignee. US Pat. No. 4615891. Oct. 1, 1986.

        • Prosky L.
        • Asp N.G.
        • Schweizer T.F.
        • Furda I.
        • Devries J.W.
        Determination of insoluble, soluble, and total dietary fiber in foods and food products: interlaboralory study.
        J. Assoc. Offic. Anal. Chem. 1988; 71: 1017
        • Robertson J.B.
        • Van Soest P.J.
        The detergent system of analysis and its application to human foods.
        in: James W.P.T. Theander O. The analysis of dietary fiber in foods. Marcel Dekker, New York, NY1981: 123
        • Schaller D.
        Analysis of cereal products and ingredients.
        Cereal Foods World. 1976; 21: 426
        • Sills V.E.
        • Wallace G.M.
        A semi-micro neutral detergent fibre method for cereal products. Vol. 2. Bull. R. Soc. N.Z. Massey Univ., Palmerston North, N.Z1982
        • Smith D.
        The nonstructural carbohydrates.
        in: Butler O.W. Bailey R.W. Chemistry and biochemistry of herbage. Academic Press, London, Engl.1973: 106
        • Sniffen C.J.
        • Russell J.B.
        • Van Soest P.J.
        The influence of carbon source, nitrogen source and growth factors on rumen microbial growth.
        in: Proc. Cornell Nutr. Conf. Feed Manuf., Syracuse, NY1983: 26
        • Strobel H.J.
        • Russell J.B.
        Effect of pH and energy spilling on bacterial protein synthesis by carbohydrate-limited cultures of mixed rumen bacteria.
        J. Dairy Sci. 1986; 69: 2941
        • Van Keulen J.
        • Young B.A.
        Evaluation of acid insoluble ash as a natural marker in minerals digestibility studies.
        J. Anim. Sci. 1978; 44: 282
        • Van Soest P.J.
        Use of detergents in the analysis of fibrous feeds. II A rapid method for the determination of fiber and lignin.
        J. Assoc. Offic. Anal. Chem. 1963; 46: 829
        • Van Soest P.J.
        Collaborative study of acid detergent fiber and lignin.
        J. Assoc. Offic. Anal. Chem. 1973; 56: 781
        • Van Soest P.J.
        Nutritional ecology of the ruminant. Comstock.
        Cornell Univ. Press, Ithaca, NY1982
        • Van Soest P.J.
        Soluble carbohydrates and the non-fiber components of feeds.
        Large Anim. Vet. 1987; 42: 44
        • Van Soest P.J.
        Fibre utilization.
        in: Proc. 26th Nutr. Conf. Feed Manuf. Dep. Anim. Sci., Univ. Guelph. Can. Feed Ind. Assoc., Guelph, ON, Can1990: 110
        • Van Soest P.J.
        • Fox D.G.
        • Mertens D.R.
        • Sniffen C.J.
        Discounts for net energy and protein-fourth revision.
        in: Proc. Cornell Nutr. Conf. Feed Manuf., Cornell Univ., Ithaca, NY1984: 121
        • Van Soest P.J.
        • Jones L.H.P.
        Analysis and classification of dietary fibre.
        in: Bratter P. Schramel P. Trace element analytical chemistry in medicine and biology. Walter de Gruyter & Co., New York, NY1988: 351
        • Van Soest P.J.
        • Robertson J.B.
        Chemical and physical properties of dietary fibre.
        in: Proc. Miles Symp. Nutr. Soc. Can, Halifax, NS, Can1976: 13
        • Van Soest P.J.
        • Robertson J.B.
        Analysis of forages and fibrous foods. AS 613 Manual.
        Dep. Anim. Sci., Cornell Univ., Ithaca, NY1985
        • Van Soest P.J.
        • Sniffen C.J.
        Nitrogen fractions in NDF and ADF.
        in: Proc. Dist Feed Conf. 39, Cincinnati, OH1984: 73
        • Van Soest P.J.
        • Wine R.H.
        Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell wall constituents.
        J. Assoc. Offic. Anal. Chem. 1967; 50: 50
        • Wainman F.W.
        • Dewey P.J.S.
        • Boyne A.W.
        Compound feedingstuffs for ruminants.
        Feedingstuffs Evaluation Unit, 3rd Rep., Rowett Res. Ihstit., Bucksburn, Aberdeen, Scotland1981
        • Welch J.G.
        Rumination, particle size and passage from the rumen.
        J. Anim. Sci. 1982; 54: 885
        • Welch J.G.
        • Smith A.M.
        Influence of forage quality on rumination time in sheep.
        J. Anim. Sci. 1969; 28: 813