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Article| Volume 76, ISSUE 2, P630-636, February 1993

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Genetic Polymorphism of Milk Proteins in Hungarian Spotted and Hungarian Grey Cattle: A Possible New Genetic Variant of β-Lactoglobulin

  • M. Baranyi
    Affiliations
    Agricultural Biotechnology Center, Institute for Animal Sciences, Szent-Györgyi Albert utca 4, H-2101 Gödöllö, Hungary
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  • Z.S. Bösze
    Affiliations
    Agricultural Biotechnology Center, Institute for Animal Sciences, Szent-Györgyi Albert utca 4, H-2101 Gödöllö, Hungary
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  • J. Buchberger
    Affiliations
    Süddeutsche Versuchs- und Forschungsanstalt, für Milchwirtschaft, Technische Universität München, lnstitut für Chemie und Physik, Vöttinger Straβe 45, D-8050 Freising-Weihenstephan, Germany
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  • I. Krause
    Affiliations
    Süddeutsche Versuchs- und Forschungsanstalt, für Milchwirtschaft, Technische Universität München, lnstitut für Chemie und Physik, Vöttinger Straβe 45, D-8050 Freising-Weihenstephan, Germany
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Open ArchiveDOI:https://doi.org/10.3168/jds.S0022-0302(93)77384-1
Genetic Polymorphism of Milk Proteins in Hungarian Spotted and Hungarian Grey Cattle: A Possible New Genetic Variant of β-Lactoglobulin
Previous ArticleEffects of Maternal Lineages Grouped by Mitochondrial Genotypes on Milk Yield and Composition1
Next ArticleSingle Gene Control of Glutathione Concentration in Blood and Its Association with Yield Traits in Lactating Holstein Cows
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      Abstract

      Genetic variants of αs1-, β-, and κ-caseins and of β-lactoglobulin were studied with PAGE and isoelectric focusing in milk samples of 101 Hungarian Spotted and 120 Hungarian Grey cows. Gene frequencies and genotypic frequencies were estimated. Significant differences in gene frequencies between the two breeds were observed at the κ-casein and β-lactoglobulin locus. The variants β-casein C, κ-casein C, and β-lactoglobulin D were not found in Hungarian Grey cows. On immobilized pH gradient, isoelectric focusing gels containing carrier ampholytes, a new unidentified protein band appeared near β-lactoglobulin B in about 10% of the milk samples of Hungarian Grey cattle.

      Key words

      Abbreviation key:

      CA-IEF (isoelectric focusing in the presence of carrier ampholytes), CA-IPG-IEF (isoelectric focusing in immobilized pH gradients in the presence of carrier ampholytes), CN (casein (used with αs1-, αs2-, β-, and κ-)), HG (Hungarian Grey), HS (Hungarian Spotted), LG (lactoglobulin (used with β-))

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      Article info

      Publication history

      Accepted: October 2, 1992
      Received: May 26, 1992

      Identification

      DOI: https://doi.org/10.3168/jds.S0022-0302(93)77384-1

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      © 1993 American Dairy Science Association. Published by Elsevier Inc.

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