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Article| Volume 79, ISSUE 5, P813-821, May 1996

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Interaction Among Bovine Somatotropin, Insulin, and Gonadotropins on Steroid Production by Bovine Granulosa and Thecal Cells1

  • Author Footnotes
    2 To whom reprint requests should be sent.
    L.J. Spicer
    Footnotes
    2 To whom reprint requests should be sent.
    Affiliations
    Department of Animal Science, Oklahoma State University, Stillwater 74078
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  • R.E. Stewart
    Affiliations
    Department of Animal Science, Oklahoma State University, Stillwater 74078
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  • Author Footnotes
    1 Approved for publication by the director of the Oklahoma Agricultural Experiment Station. This research was supported under Project H-2088 and Project Number HR4-032 from the Oklahoma Center for the Advancement of Science and Technology.
    2 To whom reprint requests should be sent.
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      Abstract

      The objective of the present study was to determine the interactions among bST, insulin, and gonadotropins on steroid production by granulosa and thecal cells from bovine follicles. Basal production of estradiol by granulosa cells from small (1 to 5 mm) and large (≥8 mm) follicles (expressed as picograms of estradiol per 105 cells per 24 h) was not affected by 50 or 300 ng/ml of bST, but 300 ng/ml of bST inhibited estradiol production that was induced by FSH plus insulin in cells from small and large follicles. Progesterone production and proliferation by granulosa cells from large follicles were not affected by 3 to 100 ng/ml of bST. In cultures of thecal cells that exhibited a >3-fold increase in androstenedione production induced by LH, 3 to 30 ng/ml of bST further increased androstenedione production by 29 to 42%) but cell proliferation and progesterone production were unaffected by bST. In cultures of thecal cells that exhibited a <2-fold increase in androstenedione production induced by LH, 3 to 30 ng/ml of bST inhibited androstenedione production by 32 to 33% and inhibited cell proliferation by 9 to 13%, but progesterone was unaffected by bST. In summary, only pharmacologic doses of bST inhibited estradiol production by granulosa cells, but physiologic doses of bST altered androstenedione production by thecal cells, which indicated that bST might not have an important role in granulosa cell function but might play a role in thecal cell function in cattle.

      Key words

      Abbreviation key:

      FCS (fetal calf serum)

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