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Field Trials of a Vaccine Against Bovine Mastitis. 1. Evaluation in Heifers

  • Author Footnotes
    1 Departamento de Patología Animal, Facultad de Agronomía y Veterinaria.
    José A. Giraudo
    Footnotes
    1 Departamento de Patología Animal, Facultad de Agronomía y Veterinaria.
    Affiliations
    Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
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  • Author Footnotes
    2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales.
    ,
    Author Footnotes
    3 To whom correspondence should be addressed.
    Aldo Calzolari
    Footnotes
    2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales.
    3 To whom correspondence should be addressed.
    Affiliations
    Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
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  • Author Footnotes
    2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales.
    Horacio Rampone
    Footnotes
    2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales.
    Affiliations
    Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
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  • Author Footnotes
    1 Departamento de Patología Animal, Facultad de Agronomía y Veterinaria.
    Alberto Rampone
    Footnotes
    1 Departamento de Patología Animal, Facultad de Agronomía y Veterinaria.
    Affiliations
    Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
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  • Author Footnotes
    2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales.
    Ana T. Giraudo
    Footnotes
    2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales.
    Affiliations
    Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
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  • Author Footnotes
    2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales.
    Cristina Bogni
    Footnotes
    2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales.
    Affiliations
    Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
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  • Author Footnotes
    1 Departamento de Patología Animal, Facultad de Agronomía y Veterinaria.
    Alejandro Larriestra
    Footnotes
    1 Departamento de Patología Animal, Facultad de Agronomía y Veterinaria.
    Affiliations
    Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
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  • Author Footnotes
    2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales.
    ,
    Author Footnotes
    4 Centro de Estudios Farmacológicos y Botá nicos, Serrano 665, 1414 Capital Federal, Argentina.
    Rosa Nagel
    Footnotes
    2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales.
    4 Centro de Estudios Farmacológicos y Botá nicos, Serrano 665, 1414 Capital Federal, Argentina.
    Affiliations
    Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
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  • Author Footnotes
    1 Departamento de Patología Animal, Facultad de Agronomía y Veterinaria.
    2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales.
    3 To whom correspondence should be addressed.
    4 Centro de Estudios Farmacológicos y Botá nicos, Serrano 665, 1414 Capital Federal, Argentina.
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      Abstract

      A vaccine was developed against bovine mastitis based on inactivated, highly encapsulated Staphylococcus aureus cells; a crude extract of Staph. aureus exopolysaccharides; and inactivated, unencapsulated Staph. aureus and Streptococcus spp. cells. This vaccine was tested on 30 heifers during a 7-mo period. The 30 heifers were randomly assigned to three groups of 10 heifers each. The prepartum group received two injections of the vaccine at 8 and 4 wk before calving, and the postpartum group received two injections at 1 and 5 wk after calving. The control group received two injections of a placebo at 8 and 4 wk before calving. The vaccine or the placebo was administered subcutaneously in the brachiocephalicus muscle of the neck. The frequencies of intramammary infections caused by Staph. aureus were reduced from 18.8% for heifers in the control group to 6.7 and 6.0% for heifers in the prepartum and post-partum groups, respectively. This protective effect was maintained for at least 6 mo. The relative risk of mastitis caused by Staph. aureus was 0.31 and 0.28 for heifers in the prepartum and postpartum groups, respectively, compared with that for heifers in the control group. The results of the trial indicated the effectiveness of the vaccine in decreasing the incidence of intramammary infections caused by Staph. aureus. A slight but nonsignificant increase occurred in fat production in the milk of vaccinated cows. The vaccine had no observable effect on somatic cell count or streptococcal infections.

      Key words

      Abbreviation Key:

      CNS (coagulase-negative staphylococci)

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