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Article| Volume 80, ISSUE 8, P1512-1519, August 1997

Bacteriophage Resistance in Lactococcus lactis Engineered by Replacement of a Gene for a Bacteriophage Receptor

  • Author Footnotes
    1 Present address: Department of Cancer Biology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157.
    K.C. Garbutt
    Footnotes
    1 Present address: Department of Cancer Biology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157.
    Affiliations
    Department of Microbiology, Nash Hall 220, and The Center for Gene Research and Biotechnology, Oregon State University, Corvallis 97331-3804
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  • J. Kraus
    Affiliations
    Department of Microbiology, Nash Hall 220, and The Center for Gene Research and Biotechnology, Oregon State University, Corvallis 97331-3804
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  • B.L. Geller
    Correspondence
    Corresponding author.
    Affiliations
    Department of Microbiology, Nash Hall 220, and The Center for Gene Research and Biotechnology, Oregon State University, Corvallis 97331-3804

    Department of Microbiology, Nash Hall 220, and The Western Dairy Center, Utah State University, Logan 84322
    Search for articles by this author
  • Author Footnotes
    1 Present address: Department of Cancer Biology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157.
Open ArchiveDOI:https://doi.org/10.3168/jds.S0022-0302(97)76080-6
Bacteriophage Resistance in Lactococcus lactis Engineered by Replacement of a Gene for a Bacteriophage Receptor
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      Abstract

      The objective of this study was to construct a food-grade, phage-resistant strain of Lactococcus lactis by replacing a specific chromosomal gene with an allele that had been mutated in vitro. Lactococcus lactis contains a chromosomal gene (pip) that is required for infection by bacteriophages of the c2 species. A nonsense mutation in pip was constructed in vitro. The wild-type pip on the chromosome of strain LM2301 was exchanged for the mutated pip. The exchange left no antibiotic resistance genes or nonlactococcal DNA in the engineered strain (JK101). JK101 was resistant to the same phages as a strain that contains a spontaneous mutation in pip. JK101 grew as well as the pip+ isogenic strain did in minimal or rich media.

      Key words

      Abbreviation Key:

      M17G (M17 growth medium plus glucose), M17G ery (M17G plus erythromycin), PCR (polymerase chain reaction)

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      Article info

      Publication history

      Accepted: February 18, 1997
      Received: October 7, 1996

      Identification

      DOI: https://doi.org/10.3168/jds.S0022-0302(97)76080-6

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      © 1997 American Dairy Science Association. Published by Elsevier Inc.

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