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Article| Volume 80, ISSUE 8, P1561-1568, August 1997

Milk Plasmin During Bovine Mammary Involution That Has Been Accelerated by Estrogen1

  • Author Footnotes
    2 Present address: Rua Goias, 1782, Centro-Avare, SP 18.701-210, Brazil.
    F. Athie
    Footnotes
    2 Present address: Rua Goias, 1782, Centro-Avare, SP 18.701-210, Brazil.
    Affiliations
    Department of Dairy and Poultry Sciences, University of Florida, Gainesville 32611
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  • Author Footnotes
    3 Reprint requests.
    K.C. Bachman
    Footnotes
    3 Reprint requests.
    Affiliations
    Department of Dairy and Poultry Sciences, University of Florida, Gainesville 32611
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  • H.H. Head
    Affiliations
    Department of Dairy and Poultry Sciences, University of Florida, Gainesville 32611
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  • M.J. Hayen
    Affiliations
    Department of Dairy and Poultry Sciences, University of Florida, Gainesville 32611
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  • C.J. Wilcox
    Affiliations
    Department of Dairy and Poultry Sciences, University of Florida, Gainesville 32611
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  • Author Footnotes
    1 Florida Agricultural Experiment Station Journal Series Number R-05276. Mention of companies or products does not constitute endorsement by the University of Florida or the Florida Agricultural Experiment Station over similar products not mentioned.
    2 Present address: Rua Goias, 1782, Centro-Avare, SP 18.701-210, Brazil.
    3 Reprint requests.
Open ArchiveDOI:https://doi.org/10.3168/jds.S0022-0302(97)76086-7
Milk Plasmin During Bovine Mammary Involution That Has Been Accelerated by Estrogen1
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      Abstract

      The purpose of this study was to evaluate whether the plasminogen and plasmin system within bovine mammary secretions was influenced by an estrogen treatment that was used to accelerate mammary tissue involution. Holstein cows were injected with 4 ml of ethanol excipient (n = 21) or 15 mg of estradiol-17β (n = 23) on each of the 4 d that preceded final milk removal. Dates of final milk removal (d 0) were designated as 60 d prior to expected dates of calving. Each mammary quarter was sampled once to collect secretions that corresponded to d 0, 3, 11, and 25 or d 1, 7, 18, and 30 of the dry period. Concentrations of plasminogen, plasmin, and somatic cells in secretions increased earlier for treated cows than for control cows. The ratio of plasminogen to plasmin in secretions decreased earlier for treated cows than for control cows. These responses support the suggestion that the plasminogen and plasmin system is involved in the involution of bovine mammary tissue. Estrogen treatment increased the activation of plasminogen, which was evidenced by a precipitous decrease in the ratio of plasminogen to plasmin that occurred as concentrations of plasminogen and plasmin increased. The activation of plasminogen likely contributed to the increased rate of mammary tissue involution that was effected by exogenous estrogen. Endogenous estrogen secreted by the developing fetal and placental unit might mediate, in part, the gradual involution that occurs during lactation.

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      Article info

      Publication history

      Accepted: January 24, 1997
      Received: July 24, 1996

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      DOI: https://doi.org/10.3168/jds.S0022-0302(97)76086-7

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      © 1997 American Dairy Science Association. Published by Elsevier Inc.

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