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Article| Volume 82, ISSUE 11, P2402-2411, November 1999

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Changes in Milk Protein Fraction as Affected by Subclinical Mastitis

Open ArchiveDOI:https://doi.org/10.3168/jds.S0022-0302(99)75491-3
Changes in Milk Protein Fraction as Affected by Subclinical Mastitis
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      Abstract

      From cows that had both healthy quarters and quarters with subclinical mastitis [somatic cell count (SCC) 84,000 vs. 293,000/ml in bucket milk], foremilk, bucket milk, and stripping and residual milks were collected. Young milk was obtained 1.5 h later following a repeated oxytocin injection. Compared with milk from healthy quarters, milk from quarters with subclinical mastitis showed elevated SCC, plasminogen, and protein and had increased activity of n-Acetyl-β-D-glucosaminidase (NAGase) and plasmin, as well as elevated portions of whey proteins and γ-casein in the total protein. The SCC and the other mentioned parameters were also higher in the foremilk and the stripping and residual milks compared with bucket milk, independent of the udder health status; however, decreased values were found for total protein. Young milk showed an increase of SCC and n-Acetyl-β-D-glucosaminidase activity compared with bucket milk. Because of lower levels of total plasmin and γ-casein, we concluded that this young milk was newly synthesized milk containing some casein degradation products and that proteolysis of casein continued in the udder until the next milking. The n-Acetyl-β-D-glucosaminidase activity was shown to be a better indicator for subclinical mastitis and correlated better with protein degradation than did SCC.

      Key words

      Abbreviation key:

      AMC (aminomethyl-coumarin), CMT (California Mastitis Test), NAGase (n-Acetyl-β-D-glucosaminidase), PL (plasmin), PLG (plasminogen)

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      Article info

      Publication history

      Accepted: May 10, 1999
      Received: November 12, 1998

      Identification

      DOI: https://doi.org/10.3168/jds.S0022-0302(99)75491-3

      Copyright

      © 1999 American Dairy Science Association. Published by Elsevier Inc.

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