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Changes in Milk Protein Fraction as Affected by Subclinical Mastitis

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      Abstract

      From cows that had both healthy quarters and quarters with subclinical mastitis [somatic cell count (SCC) 84,000 vs. 293,000/ml in bucket milk], foremilk, bucket milk, and stripping and residual milks were collected. Young milk was obtained 1.5 h later following a repeated oxytocin injection. Compared with milk from healthy quarters, milk from quarters with subclinical mastitis showed elevated SCC, plasminogen, and protein and had increased activity of n-Acetyl-β-D-glucosaminidase (NAGase) and plasmin, as well as elevated portions of whey proteins and γ-casein in the total protein. The SCC and the other mentioned parameters were also higher in the foremilk and the stripping and residual milks compared with bucket milk, independent of the udder health status; however, decreased values were found for total protein. Young milk showed an increase of SCC and n-Acetyl-β-D-glucosaminidase activity compared with bucket milk. Because of lower levels of total plasmin and γ-casein, we concluded that this young milk was newly synthesized milk containing some casein degradation products and that proteolysis of casein continued in the udder until the next milking. The n-Acetyl-β-D-glucosaminidase activity was shown to be a better indicator for subclinical mastitis and correlated better with protein degradation than did SCC.

      Key words

      Abbreviation key:

      AMC (aminomethyl-coumarin), CMT (California Mastitis Test), NAGase (n-Acetyl-β-D-glucosaminidase), PL (plasmin), PLG (plasminogen)

      References

        • Aoki Y.
        • Notsuki I.
        • Ichikawa T.
        Variation in patterns of mastitis indicators during milking in relation to infectious status.
        Anim. Sci. Technol. 1992; 63: 728-735
        • Aoki Y.
        • Notsuki I.
        • Nakano T.
        • Ichikawa T.
        Influence of infectious status of quarters on variation pattern of mastitis indicators relating to sampling time.
        Jpn. J. Zootech. Sci. 1990; 61: 1084-1094
        • Auldist M.J.
        • Coats S.
        • Rogers G.L.
        • McDowell G.H.
        Changes in the composition of milk from healthy and mastitic dairy cows during the lactation cycle.
        Aust. J. Exp. Agric. 1995; 35: 427-436
        • Auldist M.J.
        • Coats S.
        • Sutherland B.J.
        • Mayes J.J.
        • McDowell G.H.
        Effects of somatic cell count and stage of lactation on raw milk composition and the yield and quality of cheddar cheese.
        J. Dairy Res. 1996; 63: 269-280
        • Barbano D.M.
        • Rasmussen R.R.
        • Lynch J.M.
        Influence of milk somatic cell count and milk age on cheese yield.
        J. Dairy Sci. 1991; 74: 369-388
        • Benslimane S.
        • Dognin-Bergeret M.J.
        • Berdague J.-L.
        • Gaudemer Y.
        • Berdague J.L.
        Variation with season and lactation of plasmin and plasminogen concentrations in Montbeliard cows’ milk.
        J. Dairy Res. 1990; 57: 423-435
        • Berning L.M.
        • Paape M.J.
        • Miller R.H.
        • LeDane R.A.
        Variation in N-acetyl-β-D-glucosaminidase activity and somatic cell count among various milk fractions.
        J. Dairy Sci. 1987; 70: 1054-1060
        • Collin J.C.
        • Kokelaar A.
        • Rolet-Repecaud O.
        • Delacroix-Buchet A.
        Determination of caseins in cowmilk by electrophoresis and fast protein liquid chromatography (FPLC R): Comparison of results.
        Lait. 1991; 71: 339-350
        • Donnelly W.J.
        • Barry J.G.
        Casein compositional studies. III. Changes in Irish milk for manufacturing and role of milk proteinase.
        J. Dairy Res. 1983; 50: 433-441
        • Eigel W.N.
        • Butler J.E.
        • Ernstrom C.A.
        • Farrell H.M.J.
        • Harwalkar V.R.
        • Jenness R.
        • Whitney R.M.
        Nomenclature of proteins of cow's milk: fifth revision.
        J. Dairy Sci. 1984; 67: 1599-1631
        • Holdaway R.J.
        • Holmes C.W.
        • Steffert I.J.
        A comparison of indirect methods for diagnosis of subclinical intramammary infection in lactating dairy cows. Part 1: The effects of bacterial infection, stage of lactation and age of cow on eight parameters in foremilk from individual quarters, with an initial study of differences between milk fractions.
        Aust. J. Dairy Technol. 1996; 51: 64-71
      1. International Dairy Federation. 1981. Isolation and identification of mastitis bacteria. Pages 19–27 in Laboratory Methods for Use in Mastitis Work. IDF Bull. no. 132. Int. Dairy Fed., Brussels, Belgium.

        • Isaksson A.
        • Arnarp L.
        Quantitative estimation of residual milk in bovine udders—a methodological study.
        Acta Vet. Scand. 1988; 29: 259-262
      2. Jakob, E. 1993. Beziehungen zwischen dem genetischen Polymorphismus der Milchproteine und der Labfähigkeit von Milch. Ph.D. Diss., Swiss Fed. Inst. Technol., Zurich, Switzerland.

        • Kielwein G.
        Leifaden der Milchkunde und Milchhygiene.
        Paul Parey, Berlin, Germany1994
        • Knight C.H.
        • Hirst D.
        • Dewhurst R.J.
        Milk accumulation and distribution in the bovine udder during the interval between milkings.
        J. Dairy Res. 1994; 61: 167-177
        • Le Roux Y.
        • Colin O.
        • Laurent F.
        Proteolysis in samples of quarter milk with varying somatic cell counts: 1. Comparison of some indicators of endogenous proteolysis in milk.
        J. Dairy Sci. 1995; 78: 1289-1297
        • Mattila T.
        • Pyorala S.
        • Sandholm M.
        Comparison of milk antitrypsin, albumin, N-acetyl-β-D-glucosaminidase, somatic cells and bacteriological analysis as indicators of bovine subclinical mastitis.
        Vet. Res. Comm. 1986; 10: 113-124
        • Pfeilsticker H.U.
        • Bruckmaier R.M.
        • Blum J.W.
        Cisternal milk in the dairy cow during lactation and after preceding teat stimulation.
        J. Dairy Res. 1996; 63: 509-515
        • Politis I.
        • Lachance E.
        • Block E.
        • Turner J.D.
        Plasmin and plasminogen in bovine milk: a relationship with involution?.
        J. Dairy Sci. 1989; 72: 900-906
        • Politis I.
        • Ng-Kwai-Hang K.F.
        Association between somatic cell count of milk and cheese-yielding capacity.
        J. Dairy Sci. 1988; 71: 1720-1727
        • Politis I.
        • Ng-Kwai-Hang K.F.
        • Giroux R.N.
        Environmental factors affecting plasmin activity in milk.
        J. Dairy Sci. 1989; 72: 1713-1718
        • Reneau J.K.
        Effective use of dairy herd improvement somatic cell counts in mastitis control.
        J. Dairy Sci. 1986; 69: 1708-1720
        • Richardson B.C.
        • Pearce K.N.
        The determination of plasmin in dairy products.
        N. Z. J. Dairy Sci. Technol. 1981; 16: 209-220
        • Rogers S.A.
        • Mitchell G.E.
        The relationship between somatic cell count, composition and manufacturing properties of bulkmilk 6. Cheddar cheese and skimmilk yoghurt.
        Aust. J. Dairy Technol. 1994; 49: 70-74
        • Rogers S.A.
        • Slattery S.L.
        • Mitchell G.E.
        • Hirst P.A.
        • Grieve P.A.
        The relationship between somatic cell count, composition and manufacturing properties of bulk milk. III. Individual proteins. Aust.
        J. Dairy Technol. 1989; 44: 49-52
      3. SAS®. 1995. User's Guide, Release 6.10. SAS Inst., Inc., Cary.

        • Schaar J.
        Plasmin activity and proteose-peptone content of individual milks.
        J. Dairy Res. 1985; 52: 369-378
        • Schaar J.
        • Funke H.
        Effect of subclinical mastitis on milk plasminogen and plasmin compared with that on sodium, antitrypsin and N-acetyl-β-D-glucosaminidase.
        J. Dairy Res. 1986; 53: 515-528
        • Schalm O.W.
        • Carroll E.J.
        • Jain N.C.
        Bovine Mastitis.
        Lea & Febiger, Philadelphia, PA1971 (Pages 94–127)
        • Smith K.L.
        Standards for somatic cells inmilk: physiological and regulatory.
        IDF Mastitis Newsletter. 1995; 144/21: 7-9
      4. Uehlinger, P. 1999. Untersuchungen über die Effektivität einer zusätzlichen parenteralen Applikation von Antibiotika bei der Behandlung von chronisch-subklinischen Mastitiden. Vet. Med. Diss., Univ. Zurich, Switzerland.

      5. Urech, E. 1998. Veränderungen bei den Milchproteinen verursacht durch subklinische Euterentzändungen. Ph.D. Diss., Swiss Fed. Inst. Technol., Zurich, Switzerland.

        • Verdi R.J.
        • Barbano D.M.
        Preliminary investigation of the properties of somatic cell proteases.
        J. Dairy Sci. 1988; 71: 534-538
        • Verdi R.J.
        • Barbano D.M.
        • Dellavalle M.E.
        • Senyk G.F.
        Variability in true protein, casein, nonprotein nitrogen, and proteolysis in high and low somatic cellmilks.
        J. Dairy Sci. 1987; 70: 230-242
        • Wendt K.
        • Bostedt H.
        • Mielke H.
        • Fuchs H.W.
        Euterund Gesäugekrankheiten.
        Gustav Fischer Verlag, Jena, Germany1994
        • Westermeier R.
        Elektrophorese-Praktikum.
        VCHVerlagsgesellschaft, Weinheim, Germany1990
        • Wilde C.J.
        • Kerr M.A.
        • Calvert D.T.
        Intracellular degradation of newly synthesized casein in perfused rat mammary gland.
        Exp. Physiol. 1991; 76: 533-538
        • Williams D.J.
        • Marschke R.J.
        • Nottingham S.M.
        • Kitchen B.J.
        Effects of stage of lactation, number of lactations and dry period on N-acetyl-β-D-glucosaminidase levels and somatic cell count in bovine milk.
        Aust. J. Dairy Technol. 1991; 46: 43-45
        • Yamamoto M.
        • Kume T.
        • Nakano M.
        • Obara Y.
        • Kudo T.
        • Ichikawa T.
        • Notsuki I.
        Automatic measurement of electrical conductivity for the detection of bovine mastitis.
        Kieler Milchwirtsch. Forsch.-Ber. 1985; 37: 364-369